Dataset Information

A Comparative Liver Label-Free Liquid Chromatography-Mass Spectrometry Proteomic Profile Highlights Putative Metabolic Differences Between two Pig Breeds

ABSTRACT: Pig breeds have different attitude to traits like growth rate, carcass composition and reproduction parameters as well as other traits. These traits considered as external traits or end phenotypes are the outcome of complex biological processes and interactions. The main goal of pig breeding programs and the basis for crossbreeding is finding a balance between these traits. In pig production, Large White and Duroc breeds are commonly used to optimise respectively fertility and growth ability and differ on several production traits, indeed the first breed as a high fertility characters whereas Duroc is used as terminal sire for her growth performance and good carcass quality traits. In this study, we have used a quantitative label-free LC-MS proteomics approach to characterise and compare the liver proteome of two heavy Italian pig breeds, Italian Duroc and Italian Large White to identify difference due to their different genetic background. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703094.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Liver

SUBMITTER: Alessio Di Luca

LAB HEAD: Luca Fontanesi

PROVIDER: PXD009771 | Pride | 2018-09-13

REPOSITORIES: Pride

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			Action	DRS
	20171108_ADL_10A_8ul_CHIP_400fmolGFP1_01.mgf	Mgf
	20171108_ADL_10A_8ul_CHIP_400fmolGFP1_01.mzXML	Mzxml
	20171108_ADL_10A_8ul_CHIP_400fmolGFP1_01.mzid.gz	Mzid
	20171108_ADL_10A_8ul_CHIP_400fmolGFP1_01.pride.mgf.gz	Mgf
	20171108_ADL_10A_8ul_CHIP_400fmolGFP1_01.pride.mztab.gz	Mztab

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A comparative analysis of label-free liquid chromatography-mass spectrometry liver proteomic profiles highlights metabolic differences between pig breeds.

Bovo Samuele S Di Luca Alessio A Galimberti Giuliano G Dall'Olio Stefania S Fontanesi Luca L

PloS one 20180912 9

The liver is a complex organ governing several physiological processes that define biological mechanisms affecting growth, feed efficiency and performance traits in all livestock species, including pig. Proteomics may contribute to a better understanding of the relationship between liver functions and complex production traits in pigs and to characterize this species as biomedical model. This study applied, for the first time, a label-free liquid chromatography-mass spectrometry (LC-MS) proteomi ...[more]

PMID: 30208024

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