Dataset Information

Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies

ABSTRACT: Nephronophthisis is one of the leading genetic causes of end-stage renal disease in childhood. Early diagnostics and prognostics for nephronophthisis are currently limited. We aimed to identify non-invasive protein biomarkers for nephronophthisis in urinary extracellular vesicles. Extracellular vesicles were isolated from urine of 12 patients with a nephronophthisis-related ciliopathy and 12 age- and gender-matched controls, followed by in-depth label-free LC-MS/MS proteomics analysis of gel fractionated extracellular vesicles proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

DISEASE(S): Nephronophthisis

SUBMITTER: Sander Piersma

LAB HEAD: Connie Ramona Jimenez

PROVIDER: PXD009784 | Pride | 2018-08-06

REPOSITORIES: Pride

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			Action	DRS
	MaxQuantzippedFolder.zip	Other
	QE2_160920_OPL4024_MFS_nephro_urine_exo_A1.raw	Raw
	QE2_160920_OPL4024_MFS_nephro_urine_exo_A2.raw	Raw
	QE2_160920_OPL4024_MFS_nephro_urine_exo_A3_161012104553.raw	Raw
	QE2_160920_OPL4024_MFS_nephro_urine_exo_A4.raw	Raw

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Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies.

Stokman Marijn F MF Bijnsdorp Irene V IV Schelfhorst Tim T Pham Thang V TV Piersma Sander R SR Knol Jaco C JC Giles Rachel H RH Bongers Ernie M H F EMHF Knoers Nine V A M NVAM Lilien Marc R MR Jiménez Connie R CR Renkema Kirsten Y KY

Journal of proteomics 20180730

Nephronophthisis is one of the leading genetic causes of end-stage renal disease in childhood. Early diagnostics and prognostics for nephronophthisis are currently limited. We aimed to identify non-invasive protein biomarkers for nephronophthisis in urinary extracellular vesicles. Extracellular vesicles were isolated from urine of 12 patients with a nephronophthisis-related ciliopathy and 12 age- and gender-matched controls, followed by in-depth label-free LC-MS/MS proteomics analysis of gel fra ...[more]

PMID: 30071318

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