Dataset Information

Global quantitative proteomics of mouse brain structures reveals significant alterations in expression of proteins involved in neuronal plasticity during aging

ABSTRACT: Aging is believed to be the result of alterations of protein expression and accumulation of changes in biomolecules. Although there are numerous reports demonstrating changes in protein expression in brain during aging, only few of them describe global changes in the protein level. Here, we present a deepest quantitative proteomic analysis of three brain regions, hippocampus, cortex and cerebellum, in mice aged 1 and 12 months, using the total protein approach technique. In all the brain regions, both in young and in middle-aged animals, we identified over 6,700 proteins. We found that although the total protein expression in middle-aged brain structures is practically unaffected by aging, there are significant differences between young adult and middle-aged mice in the expression of some receptors and signaling cascade proteins proven to be significant for learning and memory formation. Our analysis demonstrates that hippocampus is the most unstable structure during natural aging and that the first symptoms of weakening of neuronal plasticity may be observed on protein level in middle-aged animals.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Brain, Liver

SUBMITTER: Jacek Wisniewski

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD009792 | Pride | 2018-07-03

REPOSITORIES: Pride

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			Action	DRS
	20160630_QEp7_JRW_SA_L_Q101.raw	Raw
	20160630_QEp7_JRW_SA_L_Q102.raw	Raw
	20160630_QEp7_JRW_SA_L_Q104.raw	Raw
	20160630_QEp7_JRW_SA_L_Q105.raw	Raw
	20160630_QEp7_JRW_SA_L_Q107.raw	Raw

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Publications

Systematic analysis of GSK-3 signaling pathways in aging of cerebral tissue.

Drulis-Fajdasz D D Rakus D D Rakus D D Wiśniewski J R JR McCubrey J A JA Gizak A A

Advances in biological regulation 20180621

Glycogen synthase kinase-3 (GSK-3) is a constitutively active kinase, involved in regulation of multiple physiological processes. In brain, changes in GSK-3 signaling are related to neurodegenerative issues, including Alzheimer's disease. Due to the wide range of GSK-3 cellular targets, a therapeutic use of the enzyme inhibitors entails significant risk of side effects. Thus, altering the ratio of specific pool of GSK-3 or specific substrates instead of changing the global activity of GSK-3 in b ...[more]

PMID: 29958836

Publication: 1/2

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