Proteomics

Dataset Information

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Proteomic profiling of human mesenchymal stem cells derived from umbilical cord or adipose tissue


ABSTRACT: We performed a proteomic profiling of human mesenchymal stem cells (hMSCs) derived from adipose tissue or umbilical cord.

INSTRUMENT(S): TripleTOF 5600, Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Umbilical Cord, Mesenchymal Cell, Adipose Tissue

SUBMITTER: Miguel Marcilla  

LAB HEAD: Fernando Corrales

PROVIDER: PXD009893 | Pride | 2018-07-11

REPOSITORIES: Pride

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Publications

Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.

Clemente Luis Felipe LF   Hernáez María Luisa ML   Ramos-Fernández Antonio A   Ligero Gertrudis G   Gil Concha C   Corrales Fernando José FJ   Marcilla Miguel M  

Journal of proteome research 20180706 12


Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labe  ...[more]

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