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A549 CD38 phosphoproteomics analysis

ABSTRACT: A549 CD38 overexpression cells phosphoproteomics analysis

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: weixuan wang

LAB HEAD: hai teng, deng

PROVIDER: PXD009917 | Pride | 2018-07-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MS160740_5C-PHOSPHO.msf	Msf
	MS160740_5C-PHOSPHO.pep.xml	Pepxml
	MS160740_5C-PHOSPHO.prot.xml	Xml
	MS160740_ps1.raw	Raw
	MS160740_ps10.raw	Raw

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1 - 5 of 17

Publications

Decreased NAD Activates STAT3 and Integrin Pathways to Drive Epithelial-Mesenchymal Transition.

Wang Weixuan W Hu Yadong Y Yang Changmei C Zhu Songbiao S Wang Xiaofei X Zhang Zhenyu Z Deng Haiteng H

Molecular & cellular proteomics : MCP 20180706 10

Nicotinamide adenine dinucleotide (NAD) plays an essential role in all aspects of human life. NAD levels decrease as humans age, and supplementation with NAD precursors plays a protective role against aging and associated disease. Less is known about the effects of decreased NAD on cellular processes, which is the basis for understanding the relationship between cellular NAD levels and aging-associated disease. In the present study, cellular NAD levels were decreased by overexpression of CD38, a ...[more]

PMID: 29980616

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Human A549 CD38, part 1 - Decreased NAD Activates STAT3 and Integrin Pathways to Drive Epithelial-Mesenchymal Transition

Project description:A SILAC labeled in-solution digestion of A549 CD38 cells.

2018-07-10 | PXD007122 | Pride

Human A549 CD38, part 2 - Decreased NAD Activates STAT3 and Integrin Pathways to Drive Epithelial-Mesenchymal Transition

Project description:SILAC-Labeled proteomic analysis of A549 CD38 human lung cancer cell

2018-07-11 | PXD007123 | Pride

YTHDF3 improves translation initiation of mRNAs with N6-methyladenosine methylation at 5’ untranslated region by facilitating eIF2AK2 recruitment in oxaliplatin-resistant colorectal cancer - RIP-seq dataset

Project description:Oxaliplatin as a first-line drug frequently causes the chemo-resistance on colorectal cancer (CRC). N6-methyladenosine (m6A) methylation has been largely acknowledged in multiple biological functions. However, the molecular mechanisms underlying the m6A methylation in modulating anticancer drug resistance in CRC are still obscure. In present study, RIP-seq was conducted to investigate the occupancy of N6-methyladenosine RNA binding protein 3 (YTHDF3) served as “readers” that can recognize m6A modification site in HCT116 cells with oxaliplatin resistance (HCT116R). Then, YTHDF3 was knockdown by siRNA in HCT116 cells with oxaliplatin resistance, and RIP-seq was further conducted to investigate m6A methylation of HCT116, HCT116R and HCT116R cells with YTHDF3 knockdown.

2022-02-04 | E-MTAB-11321 | biostudies-arrayexpress

Effect of Glucocorticoids on lncRNA and mRNA Expression Profile of the Human Bone Microcirculatory Endothelial Cells

Project description:Appropriate gene expression patterns form the basis for bone microcirculatory endothelial cellsM-bM-^@M-^Y function and bone morphology. Although previous studies have elucidated the impact of hydrocortisone on bone microcirculatory endothelial cellsM-bM-^@M-^Y specific gene expression, the exact differential transcriptomes and comprehensive gene expression profiles remain unknown. We have investigated the mRNA and lncRNA expression patterns before and after hydrocortisone administration of bone microcirculatory endothelial cells. At mRNAs level totally 518 differentially expressed genes were identified. Furthermore, we identified 73 upregulated and 166 downregulated long non-coding RNAs after administration of hydrocortisone. These RNAs appeared to be highly important to gene co-expression network. Transcriptomic analysis of bone microcirculatory endothelial cells from human samples is highly informative due to their relevance to the large number of expressed genes. Our study provides a very valuable basis for investigation of genes, regulation and their co-expression network contributing to hydrocortisone induced disorders. Two-condition experiment, hydrocortisone treated vs. untreated bone microcirculatory endothelial cells. Biological replicates: 8 control replicates, 8 treated replicates.

2015-01-01 | E-GEOD-60332 | biostudies-arrayexpress

Effect of Glucocorticoids on microRNA, lncRNA and mRNA Expression Profile of the Human Bone Microcirculatory Endothelial Cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-05 | E-GEOD-60333 | biostudies-arrayexpress

Enhanced solvent titer in the fermentation of Clostridium beijerinckii through application of metabolic controls

Project description:Clostridium beijerinckii is an anaerobic strain and well known for acetone-ethanol-butanol (ABE) fermentation using carbohydrates derived from cellulose or starch. During ABE fermentation, various byproducts are formed, mainly including acids (acetate, butyrate and lactate) and gas (hydrogen and carbon dioxide). recently, we found that Clostridium beijerinckii is able to produce a new product that had never been reported before and tightly regulated by pH and nitrogen source.

2021-12-02 | E-MTAB-11154 | biostudies-arrayexpress

Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes

Project description:In agroecosystems, a plant-usable form of nitrogen is mainly generated by legume-based biological nitrogen fixation, a process that requires phosphorus (P) as an essential nutrient. To investigate the physiological mechanism whereby phosphorus influences soybean nodule nitrogen fixation, soybean root nodules were exposed to four phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress), 31 mg/L (Normal P), 61 mg/L (High P) then proteome analysis of nodules was conducted to identify phosphorus-associated proteome changes. We found that phosphorus stress-induced ribosomal protein structural changes were associated with altered key root nodule protein synthesis profiles. Importantly, up-regulated expression of peroxidase was observed as an important phosphorus stress-induced nitrogen fixation-associated adaptation that supported two nodule-associated activities: scavenging of reactive oxygen species (ROS) and cell wall growth. In addition, phosphorus transporter (PT) and purple acid phosphatase (PAPs) were up-regulated that regulated phosphorus transport and utilisation to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules.

2022-08-11 | PXD033875 | Pride

A multi-omics dataset of human transcriptome and proteome stable reference

Project description:In recent years, the development of high-throughput omics technology has greatly promoted the development of biomedicine. However, the poor reproducibility of omics techniques limits its application. It is necessary to use standard reference materials of complex RNAs or proteins to test and calibrate the accuracy and reproducibility of omics workflows. Our study justified a omics standard reference material and reference datasets for transcriptomic and proteomics research. This helps to further standardize the workflow and data quality of omics techniques and thus promotes the application of omics technology in precision medicine.

2023-07-11 | PXD043389 | Pride

Antibody repertoire RNA-seq of BALB/c and C57BL/6J baseline mice (blood and spleen)

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2021-12-20 | E-MTAB-10286 | biostudies-arrayexpress

Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method

Project description:Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins.

2024-08-09 | PXD023315 | Pride

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