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Insulin Granule and Secreted Peptidome

ABSTRACT: Insulin peptidome, for dense core granules, crinosomes and secretome, was characterized by nLC-MS/MS.

OTHER RELATED OMICS DATASETS IN: GSE114824

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Urine, Islet Of Langerhans, Pancreas

DISEASE(S): Disease Free

SUBMITTER: Cheryl Lichti

LAB HEAD: Emil R. Unanue

PROVIDER: PXD009919 | Pride | 2018-07-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	7613_25K_12_peptides_1_1_0.mzid.gz	Mzid
	7613_25K_12_peptides_1_1_0.pride.mztab.gz	Mztab
	7613_5K_04_peptides_1_1_0.mzid.gz	Mzid
	7613_5K_04_peptides_1_1_0.pride.mztab.gz	Mztab
	7641_25k_peptides_1_1_0.mzid.gz	Mzid

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Publications

Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides.

Wan Xiaoxiao X Zinselmeyer Bernd H BH Zakharov Pavel N PN Vomund Anthony N AN Taniguchi Ruth R Santambrogio Laura L Anderson Mark S MS Lichti Cheryl F CF Unanue Emil R ER

Nature 20180718 7716

Tissue-specific autoimmunity occurs when selected antigens presented by susceptible alleles of the major histocompatibility complex are recognized by T cells. However, the reason why certain specific self-antigens dominate the response and are indispensable for triggering autoreactivity is unclear. Spontaneous presentation of insulin is essential for initiating autoimmune type 1 diabetes in non-obese diabetic mice<sup>1,2</sup>. A major set of pathogenic CD4 T cells specifically recognizes the 1 ...[more]

PMID: 30022165

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MHC-II peptidome of the CNS reveals endogenous guardian peptides

Project description:MHC-II peptidome of the CNS reveals endogenous guardian peptides

| PRJNA1004626 | ENA

Reanalysis of NOD mouse insulin granule and secreted peptidome data

Project description:Data from a previous publication from our lab (Nature 2018 Aug; 560 (7716): 107-111) were reanalyzed by performing a multiround search against a Uniprot-Mouse database followed by an in silico database of hybrid insulin peptides.

2020-03-11 | PXD015726 | Pride

Proteome changes of fibroblasts and endothelial cells upon incubation with human cytomegalovirus dense bodies

Project description:A mass spectrometry-based proteomics analysis was performed to study the proteome changes upon treatment with dense bodies derived from human cytomegalovirus infection.

2023-07-24 | PXD039032 | Pride

Real-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation

Project description:Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI PRISMM-BM-. 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).The expression of HSD11B2 for the 3 experiments concerned was monitored in parallel by real time PCR which confirming significant downregulation by insulin. qPCR gene expression profiling. RNA from 3 separated experiments was collected.

2014-06-01 | E-GEOD-51677 | biostudies-arrayexpress

Comprehensive analysis of different in vitro insulin resistance models

Project description:Diet-induced obesity (DIO) predisposes individuals to insulin resistance, and adipose tissue has a major role in the disease. Insulin resistance can be induced in cultured adipocytes by a variety of treatments, but what aspects of the in vivo responses are captured by these models remains unknown. We use global RNA sequencing to investigate changes induced by TNF-a, hypoxia, dexamethasone, high insulin, and a combination of TNF-a and hypoxia, comparing the results to the changes in white adipose tissue from DIO mice. We found that different in vitro models capture distinct features of DIO adipose insulin resistance, and a combined treatment of TNF-a and hypoxia is most able to mimic the in vivo changes. Using genome-wide DNase I hypersensitivity followed by sequencing, we further examined the transcriptional regulation of TNF-a-induced insulin resistance, and we found that C/EPBM-CM-^_ key regulator of adipose insulin resistance. RNA-seq for 6 insulin resistance conditions and 2 control conditions, Dnase hypersensitivity-seq of 4 conditions and 1 control condition, ChIP-seq on p65 after TNFa treatment.

2013-10-18 | E-GEOD-35724 | biostudies-arrayexpress

BIG3 inhibits insulin granule biogenesis and insulin secretion.

Project description:While molecular regulation of insulin granule exocytosis is relatively well understood, insulin granule biogenesis and maturation and its influence on glucose homeostasis are relatively unclear. Here, we identify a novel protein highly expressed in insulin-secreting cells and name it BIG3 due to its similarity to BIG/GBF of the Arf-GTP exchange factor (GEF) family. BIG3 is predominantly localized to insulin- and clathrin-positive trans-Golgi network (TGN) compartments. BIG3-deficient insulin-secreting cells display increased insulin content and granule number and elevated insulin secretion upon stimulation. Moreover, BIG3 deficiency results in faster processing of proinsulin to insulin and chromogranin A to β-granin in β-cells. BIG3-knockout mice exhibit postprandial hyperinsulinemia, hyperglycemia, impaired glucose tolerance, and insulin resistance. Collectively, these results demonstrate that BIG3 negatively modulates insulin granule biogenesis and insulin secretion and participates in the regulation of systemic glucose homeostasis.

| S-EPMC4197882 | biostudies-literature

cold exposure peptidome of male

Project description:cold exposure alters peptidome in hippocampus ,hypothalamus and pituitary connected with microbiota in gut

2022-10-17 | PXD037471 |

Analysis of the yeast peptidome and comparison with the human peptidome

Project description:Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeast peptides are similar to those of the human peptidome in average size and amino acid composition. Inhibition of proteasome activity with either bortezomib or epoxomicin led to decreased levels of some yeast peptides, suggesting that these peptides are generated by the proteasome. Approximately 30% of the yeast peptides correspond to the N- or C-terminus of the protein; the human peptidome is also highly represented in N- or C-terminal protein fragments. Most yeast and humans peptides are derived from a subset of abundant proteins, many with functions involving cellular metabolism or protein synthesis and folding. Of the 75 yeast proteins that give rise to peptides, 24 have orthologs that give rise to human and/or mouse peptides and for some, the same region of the proteins are found in the human, mouse, and yeast peptidomes. Taken together, these results support the hypothesis that intracellular peptides may have specific and conserved biological functions.

2020-06-02 | PXD004800 | Pride

Transcription profiling of human umbilical vein endothelial cells (HUVEC) treated with VEGF-A

Project description:Angiogenesis, the formation of new capillaries by sprouting from preexisting vessels, is mainly induced by VEGF-A. To identify genes which are induced by VEGF-A in endothelial cells, HUVEC were starved and induced by VEGF-A165 for 30, 60 and 150min. RNA of induced and uninduced cells was isolated and subjected to microarray analysis using Affymetrix microarray. Experiment Overall Design: Human umbilical vein endothelial cells were grown in complete EGM-2 medium in dense culture for four days without change of medium and before the stimulation in EGM-2 medium without growth factors over night. The cells were stimulated with 100ng/ml VEGF-A165 for 30, 60 and 150 min. RNA was isolated with Trizol according to the manufacturer's instructions.

2009-10-20 | E-GEOD-15464 | biostudies-arrayexpress

Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

Project description:Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. Subjects were re-examined after four weeks of retraining. Findings: Bed rest induced insulin resistance and altered the expression of more than 4,500 genes. These changes were only partly normalized after four weeks of retraining. Pathway analyses revealed significant down-regulation of 34 pathways, predominantly those of genes associated with mitochondrial function including PPARGC1A. Despite induction of insulin resistance, bed rest resulted in a paradoxically increased response to acute insulin in the general expression of genes, particularly those involved in inflammation and endoplasmatic reticulum (ER) stress. Furthermore, bed rest changed gene expressions of several insulin resistance and diabetes candidate genes. We also observed a trend toward increased PPARGC1A DNA methylation after bed rest. Conclusions: Impaired expression of PPARGC1A and other genes involved in mitochondrial function as well as a paradoxically increased response to insulin of genes involved in inflammation and ER stress may contribute to the development of insulin resistance induced by bed rest. Lack of complete normalization of changes after four weeks of exercise retraining underscores the importance of maintaining a minimum of daily physical activity. 50 samples were collected (5 samples from 10 subjects) and included in this study. Ten technical repeats were also performed in this analysis

2010-09-21 | E-GEOD-24215 | biostudies-arrayexpress

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