Browse
Submit Data
Databases
API
Help

Dataset Information

274 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of AAEL012437 (Veneno)-interacting proteins.

ABSTRACT: To investigate the repertoire of proteins interacting with the Aedes aegypti TUDOR protein AAEL012437 (Veneno), we transiently expressed a GFP-Veneno transgene in Aag2 cells. These cells were either infected with the alphavirus Sindbis virus (SINV) or mock-infected. Subsequently, cells were lysed and processed for immunoprecipitation using GFP-TRAP beads to purify Veneno-complexes. The same approach was followed for a truncated transgene lacking the first 205 amino acids (C206).

INSTRUMENT(S): Orbitrap Fusion ETD, Q Exactive

ORGANISM(S): Aedes Aegypti Aegypti

TISSUE(S): Cell Culture

SUBMITTER: Pascal Jansen

LAB HEAD: Ronald van Rij

PROVIDER: PXD009997 | Pride | 2018-12-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20160504_Fusion1_SA_PJ_JJext_AAe_WCE_TudorGFP_12441_GFP1.raw	Raw
	20160504_Fusion1_SA_PJ_JJext_AAe_WCE_TudorGFP_12441_GFP2.raw	Raw
	20160504_Fusion1_SA_PJ_JJext_AAe_WCE_TudorGFP_12441_GFP3.raw	Raw
	20160504_Fusion1_SA_PJ_JJext_AAe_WCE_TudorGFP_12441_nonGFP1.raw	Raw
	20160504_Fusion1_SA_PJ_JJext_AAe_WCE_TudorGFP_12441_nonGFP2.raw	Raw

Items per page:

1 - 5 of 21

Similar Datasets

The midguts transcriptome of Aedes aegypti (Liverpool strain) females knock down AaMesh and feed AaMesh antibody versus those knock down GFP and feed pre-immune.

Project description:We reported the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts knock down 3, 6days, feed antibody 18h transcriptome. Comparison of the midguts transcriptome of Aedes aegypti females at two knockdown time points and one feed condition; GFP dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected GFP dsRNA AaMesh dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected AaMesh dsRNA Pre-immune-18h: 18hrs after 7day-old wild type mosquitos were fed with Pre-immune AaMesh antibody-18h: 18hrs after 7day-old wild type mosquitos were fed with AaMesh antibody

2016-03-11 | E-GEOD-79070 | biostudies-arrayexpress

System-wide profiling of RNA-binding proteins uncovers key regulators of virus infection

Project description:The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these questions, we developed ‘comparative RIC’ and applied it to cells challenged with an RNA virus, called sindbis (SINV). Over two hundred RBPs display differential interaction with RNA upon SINV infection. These alterations are mainly driven by the loss of cellular mRNAs and the emergence of viral RNA. RBPs stimulated by the infection redistribute to viral replication factories and regulate the capacity of the virus to infect. For example, ablation of XRN1 causes cells to be refractory to SINV, while GEMIN5 moonlights as a regulator of SINV gene expression. In summary, RNA availability controls RBP localisation and function in SINV-infected cells.

2019-02-21 | GSE125182 | GEO

DEAD box RNA helicase 5 and 17 are novel host factors for Sindbis virus infection.

Project description:The DEAD-box ATP-dependent RNA helicases DDX5 and DDX17 play a role in many aspects of cellular RNA biology, including metabolism, translation, splicing, transcription regulation, ribosome biogenesis, mRNA nuclear export, and miRNA processing. Moreover, both RNA helicases were found to either promote or inhibit viral replication upon several RNA virus infections. Here we show that DDX5 depletion by siRNA or CRISPR/Cas9 has a negative impact on Sindbis virus (SINV) infection at the viral protein, RNA and infectious particle level. Moreover, we demonstrate that DDX5 which is predominately nuclear in uninfected conditions, re-localizes to the cytoplasm upon infection where it interacts with the viral RNA and with the SINV capsid protein. Furthermore, proteomic analysis of DDX5 interactome in mock and SINV infected HCT116 cells confirmed its interaction with DDX17 and identified PNPT1 as a new DDX5 partner. Of note, while PNPT1 localization remains mostly unchanged in mock and infected cells, DDX17 re-localizes to the cytoplasm with DDX5 upon SINV infection and interacts with SINV capsid protein. Finally, depletion of DDX17 further reduces SINV infection in a DDX5-depleted background suggesting a cumulative proviral effect of DDX5 and 17 proteins on SINV.

2024-05-22 | PXD044867 | Pride

Detection of small RNAs generated during early infection of human HEK 293 cells by the alphavirus Sindbis virus

Project description:A time course of infection of the alphavirus Sindbis virus (SINV) was used to investigate the presence of viral specific vsRNA and the changes in miRNAs profiles in human embryonic kidney 293 cells (HEK293) by high throughput DNA sequencing. Deep sequencing of small RNAs early in SINV infection (4 and 6 hpi) showed low abundance (0.8%) of viral specific RNAs (vsRNAs) , with a random uniform distribution not typical of Dicer products, suggesting they arise from non-specific degradation. Sequencing showed little variation of cellular microRNAs (miRNAs) at 4 and 6 hpi compared to uninfected cells. Twelve miRNAs exhibiting some minor differential expression by sequencing, showed insignificant modulation by Northern blot analysis. RNA was isolated from mock infected and SINV inoculated HEK 293 cells at 4hpi and 6hpi cDNA libraries were generated for the small RNA (sRNA) content of the cells and sequenced using Illumina GA II, which yielded between 29.1M and 30.5M reads per sample

2013-12-01 | E-GEOD-46254 | biostudies-arrayexpress

Gene expression profiling in wMelPop-infected Aedes aegypti

Project description:Whole genome transcriptional profiling comparing Ae. aegypti infected with Wolbachia sp. wMelPop (PGYP1) to an uninfected Ae. aegypti control line (PGYP1.tet). The objective of the experiment was to identify genes that may be involved in the life shortening phenotype associated with wMelPop infection. Two-colour experiment; infected vs. uninfected Ae. aegypti; 4 biological replicates; 2 dye swaps.

2010-05-15 | E-GEOD-17469 | biostudies-arrayexpress

Expression analysis of Meiotic drive system in Aedes aegypti during spermatogenesis

Project description:Investigation of whole genome gene expression level changes of testes in the meiotic drive system in aedes aegypti during spermatogenesis compared to non drive strain. The meiotic drive system in Aedes aegypti causes the female determining chromosome to fragment during spermatogenesis. A six chip study using total RNA from three separately extracted non driving strain testes of Aedes aegypti and three separately extracted meiotic drive strain testes of Aedes aegypti.

2010-07-21 | E-GEOD-23039 | biostudies-arrayexpress

Zebrafish as an animal model for the antiviral RNA interference pathway

Project description:We have evaluated the possible use of zebrafish to study antiviral RNAi with sindbis virus (SINV), vesicular stomatitis virus (VSV), and nodamura virus (NoV). We find that SINV and NoV viruses induce the production of virus-derived small interfering RNAs (vsiRNAs), the hallmark of antiviral RNAi, with a preference of 22 nucleotides in length after infection of larval zebrafish. Meanwhile, the suppressor of RNAi (VSR) protein, NoV B2, may affect the accumulation of the NoV virus in zebrafish.

2023-10-30 | GSE160475 | GEO

LC-MS/MS analysis of Aedes aegypti

Project description:TMT10plex-based shotgun quantitative proteomics of three species of Aedes aegypti.

2021-10-08 | PXD025994 | Pride

Induction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus

Project description:Dengue viruses (DENV) are generally maintained in a cycle which requires horizontal transmission via their arthropod vector, Ae. Aegypti, to the vertebrate host. One important consequence of this process is the interference of these arboviruses with both invertebrate and vertebrate immune systems. While infection of vertebrates causes disease, the presence of DENV gives rise to life-long, persistent infection in mosquitoes. The results of a comparative transcriptome analysis between DENV-infected and uninfected salivary glands revealed activation of both the immune deficiency (IMD) and the Toll pathways, as well as involvement of the putative antibacterial cecropin-like peptide (AAEL000598), in controlling DENV infection in Ae. aegypti. The mature form of this peptide was found to be active against DENV and Chikungunya viruses, whereas its precursor also had a strong anti-Leishmania effect. This study is the first to establish a comparative transcriptome analysis of DENV-infected and uninfected salivary glands and demonstrates that certain DENV-induced peptides, that are part of the IMD pathway, possess broad-spectrum anti-pathogenic activity and may have therapeutic potential in human. Infectious blood meals were offered to 3-day-old, adult, female Ae. aegypti Liverpool mosquitoes using a silicone membrane feeder system (Alto et al., 2005). Human blood was combined with DENV-2 16681 to provide a blood meal titer of 5.106 plaque forming units (PFU)/ml. At different time-points after the blood meal, salivary glands were dissected in acid guanidium thiocyanate-phenol-chloroform (RNAble; Eurobio, France) or phosphate-buffered saline (PBS), and the samples were frozen at -70°C until use.

2011-03-21 | E-GEOD-21528 | biostudies-arrayexpress

Cellular microRNAs in Huh7.5.1 cells

Project description:Small RNAs play a critical role in host-pathogen interaction and microRNAs have emerged as key regulators of viral infections. Based on this evidence, we wished to contribute to this research field by identifying and characterizing cellular microRNAs with a positive or negative role on Sindbis virus (SINV) infection.

2020-02-20 | GSE136740 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data