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Acetylation in Borrelia burgdorferi

ABSTRACT: Borelia burgdorferi, the causative agent of the Lyme disease, cycles between a vector (tick) and the mammalian host. Our principal goal is to study how B. burgdorferi adapts its metabolism to colonize and survive in the tick. We established the first growth-phase related acetylome and demonstrated the role of acetylation on central metabolism regulation. We also demonstrated that acetyl phosphate is the primary acetyl donor in B. burgdorferi.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Borrelia Burgdorferi B31

SUBMITTER: Julie Hardouin

LAB HEAD: Julie Hardouin

PROVIDER: PXD010065 | Pride | 2018-09-24

REPOSITORIES: Pride

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			Action	DRS
	AckA_1_acetylation_2_01.raw	Raw
	AckA_1_acetylation_2_01_UP.msf	Msf
	AckA_2_Acetyl_1_01.msf	Msf
	AckA_2_Acetyl_1_01.raw	Raw
	AckA_3_Acetyl_1_01.msf	Msf

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Publications

Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism.

Bontemps-Gallo Sébastien S Gaviard Charlotte C Richards Crystal L CL Kentache Takfarinas T Raffel Sandra J SJ Lawrence Kevin A KA Schindler Joseph C JC Lovelace Joseph J Dulebohn Daniel P DP Cluss Robert G RG Hardouin Julie J Gherardini Frank C FC

Frontiers in microbiology 20180831

The post-translational modification of proteins has been shown to be extremely important in prokaryotes. Using a highly sensitive mass spectrometry-based proteomics approach, we have characterized the acetylome of B. burgdorferi. As previously reported for other bacteria, a relatively low number (5%) of the potential genome-encoded proteins of B. burgdorferi were acetylated. Of these, the vast majority were involved in central metabolism and cellular information processing (transcr ...[more]

PMID: 30233522

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