Proteomics

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Thylakoids of Picea abies - The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)


ABSTRACT: Analysis of the subunits composition of the thylakoids protein complexes in Picea abies (Norway spruce) by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE) and in-gel tryptic digestion of single spots.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Picea Abies

TISSUE(S): Photosynthetic Cell, Leaf

SUBMITTER: Steffen Grebe  

LAB HEAD: Eva-Mari Aro

PROVIDER: PXD010071 | Pride | 2019-04-02

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
SG_SpruceDatabaseV1_0.fasta Fasta
Supplemental_S4_2D_spots_sypro.png Other
Supplemental_S5_2Dzoom_spots_silver.png Other
Thylakoid_Picea abies_spot_1.pride.mgf.gz Mgf
Thylakoid_Picea abies_spot_10.pride.mgf.gz Mgf
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Publications

The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies.

Grebe Steffen S   Trotta Andrea A   Bajwa Azfar A AA   Suorsa Marjaana M   Gollan Peter J PJ   Jansson Stefan S   Tikkanen Mikko M   Aro Eva-Mari EM  

Journal of experimental botany 20190601 12


Pinaceae are the predominant photosynthetic species in boreal forests, but so far no detailed description of the protein components of the photosynthetic apparatus of these gymnosperms has been available. In this study we report a detailed characterization of the thylakoid photosynthetic machinery of Norway spruce (Picea abies (L.) Karst). We first customized a spruce thylakoid protein database from translated transcript sequences combined with existing protein sequences derived from gene models  ...[more]

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