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Caulobacter crescentus GsrN overexpression proteome during hyperosmotic stress

ABSTRACT: In order to better define the global regulatory effects of the sRNA, GsrN, in Caulobacter crescentus during hyperosmotic stress, we submitted soluble lysates from a gsrN over-expression stain , as well as, a wild-type sample for LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caulobacter Crescentus Cb15

SUBMITTER: Matthew Tien

LAB HEAD: Sean Crosson

PROVIDER: PXD010072 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

Coherent Feedforward Regulation of Gene Expression by Caulobacter σT and GsrN during Hyperosmotic Stress.

Tien Matthew Z MZ Stein Benjamin J BJ Crosson Sean S

Journal of bacteriology 20180910 19

GsrN is a conserved small RNA that is under transcriptional control of the general stress sigma factor, σT, and that functions as a posttranscriptional regulator of Caulobacter crescentus survival under multiple stress conditions. We have defined features of GsrN structure that determine survival under hyperosmotic stress, and we have applied transcriptomic and proteomic methods to identify regulatory targets of GsrN under hyperosmotic conditions. The 5' end of GsrN, which incl ...[more]

PMID: 30012732

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Project description:In order to elucidate the global regulatory effects of the sRNA, GsrN, in Caulobacter crescentus, we submitted soluble lysates from a gsrN knockout and over-expression stain for analysis, as well as, a wild-type sample for LC-MS/MS

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