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LC-MS/MS secretome analysis of Pleurotus ostreatus PC9 (wild type) and two transformants

ABSTRACT: The white rot fungi Pleurotus ostreatus was studied for its potential as a biological pretreatment agent, i.e- its potential for decomposing lignocellulosic biomass. P. ostreatus PC9 was manipulate to either overexpress or eliminate (by gene replacement) the transcriptional regulator CRE1, known to act as a repressor in the process of carbon catabolite repression. The parental PC9 and the two transformants were grown on microcrystalline cellulose and wheat straw (3 replicates each) and the content of the resulted secretomes was analyzed by LC-MS/MS. An extensive range of carbohydrate active enzymes (CAZymes) were affected by the modification of cre1 expression levels. The three fungi revealed also differentiation towards the carbon source used in the growth media.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pleurotus Ostreatus Pc9

SUBMITTER: Shahar Yoav

LAB HEAD: Prof. Yitzhak Hadar

PROVIDER: PXD010100 | Pride | 2018-08-06

REPOSITORIES: Pride

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			Action	DRS
	Seq53899_QE2.raw	Raw
	Seq53900_QE2.raw	Raw
	Seq53901_QE2.raw	Raw
	Seq53903_QE2.raw	Raw
	Seq53904_QE2.raw	Raw

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