Proteomics

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Nanowell-Mediated Two-Dimensional Liquid Chromatography Proteome Profiling of Mammalian Cells

ABSTRACT: Proteomic data from technical developments of a nanowell-mediated two-dimensional (2D) reversed-phase nanoflow liquid chromatography (LC) separation for in-depth proteome profiling of low-nanogram samples.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pancreas

DISEASE(S): Type 1 Diabetes Mellitus

SUBMITTER: Matthew Monroe  

LAB HEAD: Ryan Kelly

PROVIDER: PXD010150 | Pride | 2018-10-18

REPOSITORIES: Pride

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Experiment_sample_descriptions.docx Other
Nanowell_2DLC_HeLa_1000cell_Fraction_10_F12_RuiCol_111517_330bar.mgf Mgf
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Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells.

Dou Maowei M   Zhu Ying Y   Liyu Andrey A   Liang Yiran Y   Chen Jing J   Piehowski Paul D PD   Xu Kerui K   Zhao Rui R   Moore Ronald J RJ   Atkinson Mark A MA   Mathews Clayton E CE   Qian Wei-Jun WJ   Kelly Ryan T RT  

Chemical science 20180718 34

Multidimensional peptide separations can greatly increase the depth of coverage in proteome profiling. However, a major challenge for multidimensional separations is the requirement of large biological samples, often containing milligram amounts of protein. We have developed nanowell-mediated two-dimensional (2D) reversed-phase nanoflow liquid chromatography (LC) separations for in-depth proteome profiling of low-nanogram samples. Peptides are first separated using high-pH LC and the effluent is  ...[more]

PMID: 30210768

Publication: 1/2

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