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Glioblastoma cells´ response to a cancer drug

ABSTRACT: An untargeted proteomics analysis was undertaken to investigate responses to a cancer drug (survivin inhibitor) and look for possible prognostic biomarkers. A non-responcive cell line(T1454) and a sensitive cell line (T1459) (according to YM155 EC50 values) were selected (controls and treated cells). The same samples were anaysed using NMR (metabolomics).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Brain Glioblastoma Multiforme

SUBMITTER: Henriette Engen Berg

LAB HEAD: Henriette Engen Berg

PROVIDER: PXD010180 | Pride | 2019-04-10

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	IIY1_0-25kDa.msf	Msf
	IIY1_0-25kDa.raw	Raw
	IIY1_130-kDa.msf	Msf
	IIY1_130-kDa.raw	Raw
	IIY1_25-70k.raw	Raw

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Publications

Nuclear Magnetic Resonance Spectroscopy to Identify Metabolite Biomarkers of Nonresponsiveness to Targeted Therapy in Glioblastoma Tumor Stem Cells.

Hvinden Ingvild Comfort IC Berg Henriette Engen HE Sachse Daniel D Skaga Erlend E Skottvoll Frøydis Sved FS Lundanes Elsa E Sandberg Cecilie J CJ Vik-Mo Einar O EO Rise Frode F Wilson Steven Ray SR

Journal of proteome research 20190418 5

Glioblastoma is the most common and malignant brain tumor, and current therapies confer only modest survival benefits. A major obstacle is our ability to monitor treatment effect on tumors. Current imaging modalities are ambiguous, and repeated biopsies are not encouraged. To scout for markers of treatment response, we used NMR spectroscopy to study the effects of a survivin inhibitor on the metabolome of primary glioblastoma cancer stem cells. Applying high resolution NMR spectroscopy (<sup>1</ ...[more]

PMID: 30964684

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Apoptosis gene signature of Survivin and its splice variant expression in breast carcinoma

Project description:Background: Survivin was described as strongly expressed in human cancer. To date, little is known about the association between Survivin splice variants and the other apoptosis-related genes. In this study, we analyzed the apoptosis gene signature of Survivin and its variant expression in breast cancer. Methods: Expression of the 5 transcripts was measured by RT-PCR in 135 breast carcinomas. Human Apoptosis Gene Arrays were used to screen the genes that could be associated with Survivin variants. Cox survival analysis was analyzed according to the breast cancer patient outcome. Results: Significant associations between Survivin transcript and the apoptotic gene array were found. Interestingly, Survivin-3B variant showed major inverse correlations with the pro-apoptotic gene array. Overexpression of Survivin-3B strongly inhibits 5-fluorouracil/epirubicin/Cyclophosphamide induced-apoptosis in breast tumor cell lines. In breast carcinomas, uni- and multivariate analysis showed patients with high level of survivin-3B expression had a shorter overall (p=0.030 and p=0.042, respectively) and disease-free (p=0.024 and p=0.009) survival. Conclusion: Our data indicate Survivin-3B could have an anti-apoptotic activity and its expression level might be used as a prognostic marker in breast carcinoma. The expression of 96 apoptosis genes and was analyzed in 41 breast carcinomas and 1 pool of normal mammary tissues. In parallel, every survivin isoform expression was quantified by real-time quantitative PCR. Data obtained with the macro-array were correlated with survivin isoform levels detected by RT-PCR.

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Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms

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Complementary metaproteomic approaches to assess marine bacteriopankton response towards algal bloom

Project description:In this project, the metaproteome of the marine bacterioplankton was analyzed to assess its respone towards an algal bloom in the southern North Sea in spring 2010. Proteins were extracted applying two different methods: (i) applying chemical cell lysis using trifluoroethanol in combination with in-solution digest and (ii) mechanical cell lysis applying bead beating, SDS-PAGE prefractionation and in-gel digest. Both samples were analyzed by nanoLC and ESI-iontrap MS. In case of the TFE lysis samples, also nanoLC-MALDI-TOF MS was applied.

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Project description:Transcriptional profiling of human embryonic kidney 293 cells comparing transfected control pEGFP-C1 (empty vector) with pEGFP (survivin vector). One condition experiment; survivin transfected cell as test sample vs empty vector transfected cell as reference; one dye swap replicate

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NES_functional_survivin vs NES_mutated_survivin exposed or unexposed to temozolomide (TMZ)

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Apoptosis gene signature of Survivin and its splice variant expression in breast carcinoma

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