Dataset Information

Grass carp serum IgM LC-ESI-MS/MS and MALDI-MS

ABSTRACT: Glycosylation represents a major post-translational modification of proteins that can influence their structure and function.Telesot immunoglobulin M (IgM) is an especially important product of the immune system because it is the main Abs in seum and plays a critical role against defense infection. However, little is known regarding site-specific N-glycan characteristics in teleost IgM , LC-ESI-MS/MS was used to analysis deglycopeptides and glycopeptides of grass carp serum IgM, and MALDI-MS was used to analysis released carbohydrates by PNGaseF diestion of grass carp serum IgM.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ctenopharyngodon Idella

TISSUE(S): Blood Plasma

SUBMITTER: Yiling Su

LAB HEAD: Hui Geng

PROVIDER: PXD010308 | Pride | 2018-11-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GH_IgM1_201710009_WJ_100min_1.rar	Other
	GH_IgM1_201710009_WJ_100min_1.raw	Raw
	GH_IgM2_201710009_WJ_100min_1.rar	Other
	GH_IgM2_201710009_WJ_100min_1.raw	Raw
	HF01_20171017_QXH_LXY_2017YFF0205400_GIgM_F1_R1.raw	Raw

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Publications

Site-Specific N-Glycan Characterization of Grass Carp Serum IgM.

Su Yi-Ling YL Wang Bing B Hu Meng-Die MD Cui Zheng-Wei ZW Wan Jian J Bai Hao H Yang Qian Q Cui Yan-Fang YF Wan Cui-Hong CH Xiong Li L Zhang Yong-An YA Geng Hui H

Frontiers in immunology 20181114

Immunoglobulin M (IgM) is the major antibody in teleost fish and plays an important role in humoral adaptive immunity. The N-linked carbohydrates presenting on IgM have been well documented in higher vertebrates, but little is known regarding site-specific N-glycan characteristics in teleost IgM. In order to characterize these site-specific N-glycans, we conducted the first study of the N-glycans of each glycosylation site of the grass carp serum IgM. Among the four glycosylation sites, the Asn- ...[more]

PMID: 30487799

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