Proteomics

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Preeclampsia causes sex-specific transcriptional and proteomic changes in foetal erythroid cells


ABSTRACT: Preeclampsia (PE) has been associated with placental dysfunction, resulting in foetal hypoxia, accelerated erythropoiesis and increased erythroblast count in the umbilical cord blood (UCB). Although the detailed effects remain unknown, placental dysfunction can also cause inflammation, nutritional and oxidative stress in the fetus that can affect erythropoiesis. Here, we compared the expression of surface adhesion molecules and erythroid differentiation capacity of UCB hematopoietic stem/ progenitor cells (HSPCs), UCB erythroid profiles along with transcriptome and proteome of these cells between male and female foetuses from PE and normotensive pregnancies. While no significant differences were observed in UCB HSPC migration/ homing and in vitro erythroid colony differentiation, the UCB HSPC transcriptome and the proteomic profile of the in vitro differentiated erythroid cells differed between PE vs normotensive samples. Accordingly, despite absence of significant differences in the UCB erythroid populations in male or female foetuses from PE or normotensive pregnancies, transcriptional changes were observed during erythropoiesis, particularly affecting male foetuses. Pathway analysis suggested deregulation in mTORC1/AMPK signaling pathways controlling cell cycle, differentiation and protein synthesis. These results associate PE with transcriptional and proteomic changes in foetal HSPCs and erythroid cells that may underlie the higher erythroblast count in the UCB in PE.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Blood Cell

DISEASE(S): Pre-eclampsia

SUBMITTER: Egor Vorontsov  

LAB HEAD: Zahra Masoumi

PROVIDER: PXD010364 | Pride | 2019-06-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2686_SPHuman_TMT_QEHF_170807_05to24-02.msf Msf
QEHF_170807_05.raw Raw
QEHF_170807_06.raw Raw
QEHF_170807_07.raw Raw
QEHF_170807_08.raw Raw
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Publications

Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells.

Masoumi Zahra Z   Maes Gregory E GE   Herten Koen K   Cortés-Calabuig Álvaro Á   Alattar Abdul Ghani AG   Hanson Eva E   Erlandsson Lena L   Mezey Eva E   Magnusson Mattias M   Vermeesch Joris R JR   Familari Mary M   Hansson Stefan R SR  

International journal of molecular sciences 20190425 8


Preeclampsia (PE) has been associated with placental dysfunction, resulting in fetal hypoxia, accelerated erythropoiesis, and increased erythroblast count in the umbilical cord blood (UCB). Although the detailed effects remain unknown, placental dysfunction can also cause inflammation, nutritional, and oxidative stress in the fetus that can affect erythropoiesis. Here, we compared the expression of surface adhesion molecules and the erythroid differentiation capacity of UCB hematopoietic stem/pr  ...[more]

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