Proteomics

Dataset Information

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Proteomic profiling of HT1080 fibrosarcoma cells of mesenchymal or amoeboid migratory phenotype


ABSTRACT: Amoeboid and mesenchymal migration of cancer cells both contribute to metastatic spreading of tumors. To characterize proteome changes underlying the different migratory modes, we performed mass spectrometry profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition induced by either doxycycline-inducible constitutively active RhoA or dasatinib treatment. Cells were kept in three-dimensional collagen gels with or without induction for 48 hours, lysed and processed for mass spectrometry analysis. In case of the inducible RhoA system, we also performed parallel two-dimensional experiments with cells on top of a dense layer of collagen (it was not feasible with dasatinib treatment).

OTHER RELATED OMICS DATASETS IN: E-MTAB-6823

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Karel Harant  

LAB HEAD: Jan Brábek

PROVIDER: PXD010425 | Pride | 2020-04-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
D1_170107152223.raw Raw
D2_170107195536.raw Raw
D3_170108002847.raw Raw
D4_170107104914.raw Raw
D5_170108050200.raw Raw
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Publications

High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen.

Čermák Vladimír V   Gandalovičová Aneta A   Merta Ladislav L   Harant Karel K   Rösel Daniel D   Brábek Jan J  

Scientific data 20200527 1


The plasticity of cancer cell invasion represents substantial hindrance for effective anti-metastatic therapy. To better understand the cancer cells' plasticity, we performed complex transcriptomic and proteomic profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition (MAT). As amoeboid migratory phenotype can fully manifest only in 3D conditions, all experiments were performed with 3D collagen-based cultures. Two previously described approaches to induce MAT were used:  ...[more]

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