Dataset Information

The Antarctic Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of Photosystem I in response to iron

ABSTRACT: Past studies have shown that the photosynthetic apparatus of a psychrophilic alga C. sp. UWO241 is remodeled to support high rates of Photosystem I (PSI)-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range; therefore, we hypothesized that PSI restructuring in C. sp. UWO241 may reflect a strategy to survive long-term Fe-deficiency. We studied the effect of Fe availability in C. sp. UWO241, a mesophile, C. reinhardtii, and a second ELB psychrophile, Chlamydomonas sp. ICE-MDV. Under Fe-deficient conditions (2 µM Fe), abundance of the PSI reaction center protein, PsaA, as well as levels of photooxidizable P700 (ΔA820/A820) were significantly reduced in both psychrophiles relative to C. reinhardtii. In response to increased Fe, C. sp. ICE-MDV exhibited increases in PSI 77K Chl a fluorescence and ΔA820/A820 which matched that of C. reinhardtii, while C. sp. UWO241 exhibited moderate restoration of PSI function. Our results indicate that PSI functional organization in C. sp. UWO241 is unique. The unique physiological traits in PSI photochemistry exhibited by the psychrophiles may reflect adaptation to the stratified physicochemical conditions present the shallow vs. deep photic zones of a chemically Antarctic lake.

INSTRUMENT(S): LTQ

ORGANISM(S): Chlamydomonas Sp. Uwo 241

TISSUE(S): Cell Culture

SUBMITTER: XIN WANG

LAB HEAD: Xin Wang

PROVIDER: PXD010433 | Pride | 2019-03-15

REPOSITORIES: Pride

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The Antarctic psychrophiles Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron.

Cook Greg G Teufel Amber A Kalra Isha I Li Wei W Wang Xin X Priscu John J Morgan-Kiss Rachael R

Photosynthesis research 20190207 2

Chlamydomonas sp. UWO241 is a psychrophilic alga isolated from the deep photic zone of a perennially ice-covered Antarctic lake (east lobe Lake Bonney, ELB). Past studies have shown that C. sp. UWO241 exhibits constitutive downregulation of photosystem I (PSI) and high rates of PSI-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range leading us to hypothesize that the unusual PSI phenotype of C. sp. UWO241 could be a response to chronic Fe-deficiency. We studied t ...[more]

PMID: 30729447

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