Protein and metabolite composition of the Arabidopsis stress granules.

ABSTRACT: Stress granules are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Herein, we report protein composition of the Arabidopsis SGs supporting their role in plant response to stress. Moreover, and to our knowledge for the first time, we provide evidence for not only mRNAs and proteins, but also phospholipids and amino-acids accumulation in the SGs. A quarter of the identified proteins constituted known or predicted SGs components, supporting evolutionary conserved mechanism of SGs assembly and dynamics. Intriguingly, remaining proteins were enriched in key enzymes and regulators mediating plant response to stress, such as cyclin dependent kinase A (CDKA), pointing to the important role of SGs in moderating plant response to stress by selective storage and temporary inactivation of the proteins. We hypothesize that phospholipid sequestration into SGs may serve partly analogous role providing protection from the phospholipase mediated degradation occurring upon combination of heat and dark stress.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Culture

SUBMITTER: Michal Gorka  

LAB HEAD: Dr. Aleksandra Skirycz

PROVIDER: PXD010455 | Pride | 2022-03-27

REPOSITORIES: Pride