Dataset Information

Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2

ABSTRACT: Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) known to be beneficial for human health. Many of these carbohydrates are reported to have immunomodulatory properties; however the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification method introduces yeast-derived proteins reactive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS samples to Dectin-2. While OsLu purified with active charcoal and commercial GOS failed to bind to Dectin-2, we found OsLu-Sc bound to this receptor. The carbohydrate-binding incompetent mutant of Dectin-2 failed to bind to OsLu-Sc. These data suggest that OsLu-Sc introduced carbohydrate ligands for Dectin-2. In accordance with this, proteomic analysis revealed OsLu-Sc contained S. cerevisiae-derived mannoproteins. Therefore, our data highlights the importance of the purification method for OsLu, which may positively affect the bioactivity of OsLu.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Sergio Ciordia

LAB HEAD: Mar Villamiel Guerra

PROVIDER: PXD010495 | Pride | 2018-12-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170418_03_MVillamiel_EtOH.dat	Other
	20170418_03_MVillamiel_EtOH.mgf	Mgf
	20170418_03_MVillamiel_EtOH.mzid.gz	Mzid
	20170418_03_MVillamiel_EtOH.pride.mgf.gz	Mgf
	20170418_03_MVillamiel_EtOH.pride.mztab.gz	Mztab

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Publications

Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2.

Young Ian D ID Montilla Antonia A Olano Agustín A Wittmann Alexandra A Kawasaki Norihito N Villamiel Mar M

Food research international (Ottawa, Ont.) 20180730

Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) beneficial for human health including immunomodulatory properties; however, the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification introduces yeast-derived proteins reactive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS ...[more]

PMID: 30599920

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