Proteomics

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Proteome of the rodent malaria parasite Plasmodium berghei liver stage merosomes

ABSTRACT: We use the Plasmodium berghei rodent model to characterize the proteome of the final phase of liver stage development, the merosomes, packets of hepatic merozoites that bud from the host hepatocyte to initiate the blood stage of malaria. Plasmodium berghei WT ANKA strain was used to infect HepG2 hepatoma cells. Samples were fractionated by strong cation exchange, and nano-LC Orbitrap mass spectrometry was used to perform untargeted proteomic profiling of 3 biological replicates. Data was processed using MaxQuant and LFQ. Additional searches were performed to identify peptides from cleaved acetylated PEXELs (protein export elements) to identify proteins putatively exported to the host hepatocyte during liver stage development.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Anopheles Stephensi (asian Malaria Mosquito) Homo Sapiens (human) Bos Taurus (bovine) Plasmodium Berghei Anka

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD010559 | Pride | 2019-03-25

REPOSITORIES: Pride

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Pbergehei_Merosomes_SCX_Rep1_F1.raw Raw
Pbergehei_Merosomes_SCX_Rep1_F2.raw Raw
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Publications

Proteomic Analysis of <i>Plasmodium</i> Merosomes: The Link between Liver and Blood Stages in Malaria.

Shears Melanie J MJ   Sekhar Nirujogi Raja R   Swearingen Kristian E KE   Renuse Santosh S   Mishra Satish S   Jaipal Reddy Panga P   Moritz Robert L RL   Pandey Akhilesh A   Sinnis Photini P  

Journal of proteome research 20190723 9

The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We i  ...[more]

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