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Interaction of the GEF SH3BP5 and its cognate GTPase Rab11

ABSTRACT: H/D exchange analysis of the GEF SH3BP5 and its interaction with the GTPase Rab11

INSTRUMENT(S): Bruker Daltonics micrOTOF series

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke

LAB HEAD: John Edmund Burke

PROVIDER: PXD010586 | Pride | 2018-09-18

REPOSITORIES: Pride

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			Action	DRS
	01_11_2017_MJHDX30_WT_0.3_0_01_4801.d.zip	Other
	01_11_2017_MJHDX30_WT_0.3_2_0_01_4817.d.zip	Other
	01_11_2017_MJHDX30_WT_300_0_01_4803.d.zip	Other
	01_11_2017_MJHDX30_WT_300_2_0_01_4819.d.zip	Other
	01_11_2017_MJHDX30_p27_0.3_0_01_4813.d.zip	Other

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Publications

Structural determinants of Rab11 activation by the guanine nucleotide exchange factor SH3BP5.

Jenkins Meredith L ML Margaria Jean Piero JP Stariha Jordan T B JTB Hoffmann Reece M RM McPhail Jacob A JA Hamelin David J DJ Boulanger Martin J MJ Hirsch Emilio E Burke John E JE

Nature communications 20180914 1

The GTPase Rab11 plays key roles in receptor recycling, oogenesis, autophagosome formation, and ciliogenesis. However, investigating Rab11 regulation has been hindered by limited molecular detail describing activation by cognate guanine nucleotide exchange factors (GEFs). Here, we present the structure of Rab11 bound to the GEF SH3BP5, along with detailed characterization of Rab-GEF specificity. The structure of SH3BP5 shows a coiled-coil architecture that mediates exchange through a unique Rab- ...[more]

PMID: 30217979

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