Dataset Information

Development of the cell wall in alfalfa

ABSTRACT: In this study, the cell wall proteomes of 3 different regions of alfalfa stems were compared. These three regions correspond to three phases in the stem development. The sequential extraction of cell wall proteins with CaCl2, EGTA and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the three proteome patterns are characteristic for each region. In the basal stem region, proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate. Beta-amylase and ferritin likewise accumulate more in the basal stem segment. Therefore remobilization of nutrients appears to be an important process in the oldest stem segment. The intermediate and the apical region are sites of cell wall polymer remodelling, as suggested by the high abundance of proteins involved in the remodelling of the cell wall such as xyloglucan endoglucosylase, polygalacturonase non-catalytic subunit or beta-galactosidase. The most striking change between the different stem parts is however the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem which suggests a particular role of this protein during the early development of stem tissues.

INSTRUMENT(S): SCIEX TOF/TOF Series Explorer Software

ORGANISM(S): Medicago Sativa (alfalfa)

TISSUE(S): Plant Cell, Plant Stem Cell, Stem Cell, Shoot

SUBMITTER: kjell sergeant

LAB HEAD: Kjell Sergeant

PROVIDER: PXD010666 | Pride | 2019-11-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	CaCl-1013.dat-pride.xml.gz	Xml
	CaCl-1076.dat-pride.xml.gz	Xml
	CaCl-1115.dat-pride.xml.gz	Xml
	CaCl-1274.dat-pride.xml.gz	Xml
	CaCl-1276.dat-pride.xml.gz	Xml

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The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.

Sergeant Kjell K Printz Bruno B Guerriero Gea G Renaut Jenny J Lutts Stanley S Hausman Jean-Francois JF

Biology 20190819 3

In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared. Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained. This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and struc ...[more]

PMID: 31430995

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