Dataset Information

Characterization of the Sperm Proteome and Reproductive Outcomes with in Vitro Fertilization after a Reduction in Male Ejaculatory Abstinence Period

ABSTRACT: Semen samples from men after a short ejaculatory abstinence show improved sperm quality and result in increased pregnancy rates, but the underlying mechanisms remain unclear. Herein, we report that ejaculates from short (1–3 hours) compared with long (3–7 days) periods of abstinence showed increases in motile sperm count, sperm vitality, normal sperm morphology, acrosome reaction capacity, total antioxidant capacity, sperm mitochondrial membrane potential, high DNA stainability, and a decrease in the sperm DNA fragmentation index (P < 0.05). Sperm proteomic analysis showed 322 differentially expressed proteins (minimal fold change of ±1.5 or greater and P < 0.05), with 224 up-regulated and 98 down-regulated. These differentially expressed proteins are profoundly involved in specific cellular processes, such as motility and capacitation, oxidative stress, and metabolism. Interestingly, protein trimethyllysine modification was increased, and butyryllysine, propionyllysine, and malonyllysine modifications were decreased in ejaculates from a short versus long abstinence (P < 0.05). Finally, the rates of implantation, clinical pregnancy, and live births from in vitro fertilization treatments were significantly increased in semen samples after a short abstinence. Our study provides preliminary mechanistic insights into improved sperm quality and pregnancy outcomes associated with spermatozoa retrieved after a short ejaculatory abstinence

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Semen

SUBMITTER: xiaomei li

LAB HEAD: Da Li

PROVIDER: PXD010695 | Pride | 2019-02-22

REPOSITORIES: Pride

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Publications

Characterization of the Sperm Proteome and Reproductive Outcomes with <i>in Vitro</i>, Fertilization after a Reduction in Male Ejaculatory Abstinence Period.

Shen Zi-Qi ZQ Shi Bei B Wang Tian-Ren TR Jiao Jiao J Shang Xue-Jun XJ Wu Qi-Jun QJ Zhou Yi-Ming YM Cao Tie-Feng TF Du Qiang Q Wang Xiu-Xia XX Li Da D

Molecular & cellular proteomics : MCP 20180820 Suppl 1

Semen samples from men after a short ejaculatory abstinence show improved sperm quality and result in increased pregnancy rates, but the underlying mechanisms remain unclear. Herein, we report that ejaculates from short (1-3 h) compared with long (3-7 days) periods of abstinence showed increases in motile sperm count, sperm vitality, normal sperm morphology, acrosome reaction capacity, total antioxidant capacity, sperm mitochondrial membrane potential, high DNA stainability, and a decrease in th ...[more]

PMID: 30126978

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Dataset Information

Characterization of the Sperm Proteome and Reproductive Outcomes with in Vitro Fertilization after a Reduction in Male Ejaculatory Abstinence Period

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Publications

Characterization of the Sperm Proteome and Reproductive Outcomes with <i>in Vitro</i>, Fertilization after a Reduction in Male Ejaculatory Abstinence Period.

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