Dataset Information

Proteome analysis of human neutrophil granulocytes from patients with monogenic disease using data-independent acquisition

ABSTRACT: Neutrophil granulocytes are critical mediators of innate immunity and tissue regeneration. Rare diseases of neutrophil granulocytes may affect their differentiation and/or functions. However, there are very few validated diagnostic tests assessing the functions of neutrophil granulocytes in these diseases. Here, we set out to probe omics analysis as a novel diagnostic platform for patients with defective differentiation and function of neutrophil granulocytes. We analyzed highly purified neutrophil granulocytes from 68 healthy individuals and 16 patients with rare monogenic diseases. Cells were isolated from fresh venous blood (purity >99%) and used to create a spectral library covering almost 8000 proteins using strong cation exchange fractionation. Patient neutrophil samples were then analyzed by data-independent acquisition proteomics, quantifying 4154 proteins in each sample.

OTHER RELATED OMICS DATASETS IN: GSE118644

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil

DISEASE(S): Leukocyte Adhesion Deficiency,Severe Congenital Neutropenia,Chronic Granulomatous Disease

SUBMITTER: Piotr Grabowski

LAB HEAD: Juri Rappsilber

PROVIDER: PXD010701 | Pride | 2019-02-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181130_neutrophils_MCP.sne	Other
	B170603_02_HF_PG_IN_135_neutro_fracS1.raw	Raw
	B170603_04_HF_PG_IN_135_neutro_fracS2.raw	Raw
	B170603_06_HF_PG_IN_135_neutro_fracS3.raw	Raw
	B170603_08_HF_PG_IN_135_neutro_fracS4.raw	Raw

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Publications

Proteome Analysis of Human Neutrophil Granulocytes From Patients With Monogenic Disease Using Data-independent Acquisition.

Grabowski Piotr P Hesse Sebastian S Hollizeck Sebastian S Rohlfs Meino M Behrends Uta U Sherkat Roya R Tamary Hannah H Ünal Ekrem E Somech Raz R Patıroğlu Türkan T Canzar Stefan S van der Werff Ten Bosch Jutte J Klein Christoph C Rappsilber Juri J

Molecular & cellular proteomics : MCP 20190110 4

Neutrophil granulocytes are critical mediators of innate immunity and tissue regeneration. Rare diseases of neutrophil granulocytes may affect their differentiation and/or functions. However, there are very few validated diagnostic tests assessing the functions of neutrophil granulocytes in these diseases. Here, we set out to probe omics analysis as a novel diagnostic platform for patients with defective differentiation and function of neutrophil granulocytes. We analyzed highly purified neutrop ...[more]

PMID: 30630937

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