Dataset Information

Nitric oxide modulates Saccharomyces cerevisiae biofilm formation though regulating the transmembrane protein CTR1 by activating its transcriptional factor MAC1.

ABSTRACT: Biofilms with immobilized cells in industrial fermentation are beneficial. Encased in extracellular polymeric substance, cells forming biofilms are regulated by various factors. Nitric oxide (NO), as a signaling molecule, recognized as quorum sensing molecule regulating microbe biofilm formation. Regulation mechanisms of NO on bacteria biofilm have been studied extensively and deeply, while on fungus are rarely studied. In this study, we observed that low concentration of NO enhanced S. cerevisiae biofilm formation. Transcriptional and proteomic analysis revealed that transcription factor MAC1 was activated in biofilm cells under NO treatment. Overexpressed MAC1 increased yeast biofilm formation bypassing regulating the expression level of FLO11. Increased copper and iron contents in NO treated and MAC1 overexpressed cells were not responsible for increased biofilm formation. Among six downstream genes of MAC1, overexpressed CTR1 contributed yeast biofilm formation. Moreover, MAC1 and CTR1 contributed to biofilm cells ethanol resistance resulting from enhanced biofilm. The role of CTR1 protein in yeast biofilm formation may result from its hydrophobic residues in N-terminal extracellular domain. These findings suggested a NO-mediated biofilm formation mechanism that NO regulated expression levels of CTR1 through activating its transcription factor MAC1, leading enhanced biofilm formation.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: xiaomei li

LAB HEAD: Hanjie Ying

PROVIDER: PXD010751 | Pride | 2019-11-12

REPOSITORIES: pride

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Nitric oxide increases biofilm formation in <i>Saccharomyces cerevisiae</i> by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1.

Yang Leyun L Zheng Cheng C Chen Yong Y Shi Xinchi X Ying Zhuojun Z Ying Hanjie H

Biotechnology for biofuels 20190214

<h4>Background</h4>Biofilms with immobilized cells encased in extracellular polymeric substance are beneficial for industrial fermentation. Their formation is regulated by various factors, including nitric oxide (NO), which is recognized as a quorum-sensing and signal molecule. The mechanisms by which NO regulates bacterial biofilms have been studied extensively and deeply, but were rarely studied in fungi. In this study, we observed the effects of low concentrations of NO on biofilm formation i ...[more]

PMID: 30809273

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Dataset Information

Nitric oxide modulates Saccharomyces cerevisiae biofilm formation though regulating the transmembrane protein CTR1 by activating its transcriptional factor MAC1.

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Publications

Nitric oxide increases biofilm formation in <i>Saccharomyces cerevisiae</i> by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1.

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