Proteomics

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Comparative proteome analysis of serum exosomes from newborn piglets inresponse to porcine epidemic diarrhea virus infection


ABSTRACT: The immune system is thought to be fragile in the neonate, which is susceptible to pathogens. Exosomes are a type of vehicles existing in the body fluid and participate in many biological processes, especially the immune response. Inorder to investigate the roles that exosomes may play during virus infection in the neonate, porcine epidemic diarrhea virus (PEDV), a devastating enteric virus to newborn piglets, was selected for infection. Serum exosomes were then isolated from the newborn-piglets infected or mock-infected with PEDV and followed by a label-free LC-MS/MS based comparative quantitative proteomic analysis. Among 441 proteins detected in the serum exosomes, there were lots of complement proteins. The expression level of the complement C3, C6 and CFB suffered drastic changes due to PEDV infection. After the confirmation by western-blot assay, we then investigated the function of these exosomes on PEDV infection and discovered that the exosomes from mock-infected newborn piglets restricted PEDV infection but this inhibition disappeared after exosomes were heat-inactivated, suggesting that the complement is one of the key antiviral molecules. These findings will facilitate the understanding of the antiviral response of the neonate mediated by exosomes

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ssrna Viruses Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Blood Serum, Extracellular Exosome Assembly

SUBMITTER: jianing chen  

LAB HEAD: liu guangliang

PROVIDER: PXD010762 | Pride | 2019-04-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P17468_B_MOCK.raw Raw
P17468_B_MOCK_170914012859.raw Raw
P17468_B_MOCK_170914104156.raw Raw
P17468_B_PEDV.raw Raw
P17468_B_PEDV_170914034714.raw Raw
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Publications

Serum Exosomes from Newborn Piglets Restrict Porcine Epidemic Diarrhea Virus Infection.

Chen Jianing J   Jin Li L   Yan Miaomiao M   Yang Ze Z   Wang Haiwen H   Geng Shuxian S   Gong Zhenli Z   Liu Guangliang G  

Journal of proteome research 20190417 5


Exosomes are vehicles in the body fluid that participate in many biological processes, especially immune responses. In this study, we employed comparative proteome analysis to investigate the roles of serum exosomes during viral infection in neonates using porcine epidemic diarrhea virus (PEDV), a devastating enteric virus in newborn piglets, as a model virus. Serum exosomes were first isolated from newborn piglets infected with PEDV or mock-infected newborn piglets, followed by label-free LC-MS  ...[more]

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