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Comparison of bone demineralization protocols for paleoproteomics applications

ABSTRACT: Protocols for bone protein extraction adapted for radiocarbon dating were compared for their capability of being used also for paleoproteomics applications. Eight extraction protocols were tested on modern and archaeological bones. Molecular characterization of the extracts was performed by shot-gun proteomics. The effect of each extraction protocol on species identification through database searchnig was evaluated.

INSTRUMENT(S): QSTAR

ORGANISM(S): Rattus Norvegicus (rat) Bos Taurus (bovine) Mammuthus Ovis Aries Muridae Amphibia Balaenidae Mus Musculus (mouse)

TISSUE(S): Long Bone

SUBMITTER: Severine Zirah

LAB HEAD: Severine Zirah

PROVIDER: PXD010775 | Pride | 2018-12-11

REPOSITORIES: Pride

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Project description:Paleoproteomics, the study of ancient proteins, is a rapidly growing field at the intersection of molecular biology, paleontology, archaeology, paleoecology, and history. Paleoproteomics research leverages the longevity and diversity of proteins to explore fundamental questions about the past. While its origins predate the characterization of DNA, it was only with the advent of soft ionization mass spectrometry that the study of ancient proteins became truly feasible. Technological gains over the past 20 years have allowed increasing opportunities to better understand preservation, degradation, and recovery of the rich bioarchive of ancient proteins found in the archaeological and paleontological records. Growing from a handful of studies in the 1990s on individual highly abundant ancient proteins, paleoproteomics today is an expanding field with diverse applications ranging from the taxonomic identification of highly fragmented bones and shells and the phylogenetic resolution of extinct species to the exploration of past cuisines from dental calculus and pottery food crusts and the characterization of past diseases. More broadly, these studies have opened new doors in understanding past human-animal interactions, the reconstruction of past environments and environmental changes, the expansion of the hominin fossil record through large scale screening of nondiagnostic bone fragments, and the phylogenetic resolution of the vertebrate fossil record. Even with these advances, much of the ancient proteomic record still remains unexplored. Here we provide an overview of the history of the field, a summary of the major methods and applications currently in use, and a critical evaluation of current challenges. We conclude by looking to the future, for which innovative solutions and emerging technology will play an important role in enabling us to access the still unexplored "dark" proteome, allowing for a fuller understanding of the role ancient proteins can play in the interpretation of the past.

			Action	DRS
	10_BEHUM_Brown.mgf	Mgf
	10_BEHUM_Brown.pride.mgf.gz	Mgf
	10_BEHUM_MNHN.mgf	Mgf
	10_BEHUM_MNHN.pride.mgf.gz	Mgf
	10_BEHUM_MNHN.wiff	Wiff

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Comparison of bone demineralization protocols for paleoproteomics applications

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