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Analyzing Bioactive Effects of the Minor Hop Compound Xanthohumol C on Human Breast Cancer Cells Using Quantitative Proteomics

ABSTRACT: We provide label-free data-dependent acquisition proteomics data of mcf-7 breast cancer cell lines treated with the pure minor hop compounds xanthohumol and xanthohumol c.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Juergen Behr

LAB HEAD: Juergen Behr

PROVIDER: PXD010785 | Pride | 2019-03-05

REPOSITORIES: Pride

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			Action	DRS
	009606_Human_uniprot-sp_canonical_171017_reviewedentries_P057.fasta	Fasta
	BBM_049_P057_01_HEK_01.raw	Raw
	BBM_049_P057_01_HEK_02.raw	Raw
	BBM_049_P057_01_HEK_03.raw	Raw
	BBM_049_P057_01_HEK_04.raw	Raw

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Publications

Analyzing bioactive effects of the minor hop compound xanthohumol C on human breast cancer cells using quantitative proteomics.

Roehrer Simon S Stork Verena V Ludwig Christina C Minceva Mirjana M Behr Jürgen J

PloS one 20190315 3

Minor prenylated hop compounds have been attracting increasing attention due to their promising anticarcinogenic properties. Even after intensive purification from natural raw extracts, allocating certain activities to single compounds or complex interactions of the main compound with remaining impurities in very low concentration is difficult. In this study, dose-dependent antiproliferative and cytotoxic effects of the promising xanthohumol (XN) analogue xanthohumol C (XNC) were evaluated and c ...[more]

PMID: 30875365

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Microarray analysis of gene expression profiles in response to tomato leaves extract treatment in MCF-7 breast cancer cells

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Project description:Metastasis is responsible for the majority of deaths in a variety of cancer types, including breast cancer. Although several factors or biomarkers have been identified to predict the outcome of patients with breast cancer, few studies have been conducted to identify metastasis-associated biomarkers. Quantitative iTRAQ proteomics analysis was used to detect differentially expressed proteins between lymph node metastases and their paired primary tumor tissues from 23 patients with metastatic breast cancer. Immunohistochemistry was performed to validate the expression of two upregulated (EpCAM, FADD) and two downregulated (NDRG1, αB-crystallin) proteins in 190 paraffin-embedded tissue samples. These four proteins were further analyzed for their correlation with clinicopathological features in 190 breast cancer patients. We identified 637 differentially regulated proteins (397 upregulated and 240 downregulated) in lymph node metastases compared with their paired primary tumor tissues. Furthermore, bioinformatics analysis using GEO profiling confirmed the difference in the expression of EpCAM between metastases and primary tumors tissues. Two upregulated (EpCAM, FADD) and two downregulated (NDRG1, αB-crystallin) proteins were associated with the progression of breast cancer. Obviously, EpCAM plays a role in the metastasis of breast cancer cells to the lymph node. We further identified αB-crystallin as an independent biomarker to predict lymph node metastasis and the outcome of breast cancer patients.

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Identification of cytoplasmic proteins interacting with unliganded estrogen receptor α and β- in human breast cancer cells

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