Proteomic consequences of early postnatal, chronic THC exposure for the adolescent and adult hippocampus
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ABSTRACT: This study aims to identify the proteomic targets of THC in the early postnatal hippocampus of developing mice. Therefore, early postnatal C57Bl/6 mice (P5) were exposed daily and for 30 days to plant extracted THC (either 1 mg/kg or 5 mg/kg) or vehicle solution (saline with 3% Tween® 80) for the control group. All animals stayed with their mothers until P25 and after the initial drug exposure time (until P35) animals were given a drug-free resting period. The animals were sacrificed, and their hippocampus was dissected and prepared for the following proteomic analysis at either P48 or after an extended resting period at P120. We found 31 proteins to be changed after THC exposure compared to vehicle at P48 and 186 proteins showing modifications at P120. Gene ontology classification of protein targets revealed a substantial amount of proteins involved in metabolic processes of neurons after THC exposure. The results highlight the vulnerability of the developing hippocampus towards THC exposure and identify the mitochondrial as well as other cell metabolic processes as potential drug targets.
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Edit Szodorai
LAB HEAD: Univ. Prof. Dr. Tibor Harkany
PROVIDER: PXD010802 | Pride | 2020-11-09
REPOSITORIES: Pride
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