Proteomics

Dataset Information

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A photo-cleavable surfactant for top-down proteomics


ABSTRACT: We report the discovery of a photo-cleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo) that can be rapidly degraded upon UV irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to SDS and is mass spectrometry (MS)-compatible. Azo-aided top-down proteomics allowed the detection of 100-fold more unique proteoforms and enabled the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Azo is simple to synthesize in large quantity for general use as an SDS replacement.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human) Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Heart, Kidney

SUBMITTER: kyle brown  

LAB HEAD: Ying Ge

PROVIDER: PXD010825 | Pride | 2019-03-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Azo-CardiacTissue-SRandMit-2.baf Other
Azo-CardiacTissue-SRandMit.OUTPUT_TABLE Other
Azo-CardiacTissue-SRandMit.baf Other
Azo-CardiacTissue-SRandMit.msalign Other
Azo-CardiacTissue-SRandMit_2.OUTPUT_TABLE Other
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Publications

A photocleavable surfactant for top-down proteomics.

Brown Kyle A KA   Chen Bifan B   Guardado-Alvarez Tania M TM   Lin Ziqing Z   Hwang Leekyoung L   Ayaz-Guner Serife S   Jin Song S   Ge Ying Y  

Nature methods 20190415 5


We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo i  ...[more]

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