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Proteomic analysis of small and large EVs from DKs8 cells

ABSTRACT: We used iTRAQ-quantitative proteomics analysis to identify protein cargos that may contribute to different functions of exosome-enriched small extracellular vesicles (EVs) and MV-enriched large EVs derived from a colorectral cancer cell line, DKs8.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Lizandra Jimenez

LAB HEAD: Lizandra Jimenez

PROVIDER: PXD010840 | Pride | 2019-01-17

REPOSITORIES: Pride

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			Action	DRS
	LJDatarep1.xlsx	Xlsx
	LJDatarep2.xlsx	Xlsx
	LJDatarep3.xlsx	Xlsx
	ah_4223_AM_100615_microvessicles_exosomes_0mM.raw	Raw
	ah_4223_AM_100615_microvessicles_exosomes_100mM.raw	Raw

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Publications

Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs.

Jimenez Lizandra L Yu Hui H McKenzie Andrew J AJ Franklin Jeffrey L JL Patton James G JG Liu Qi Q Weaver Alissa M AM

Journal of proteome research 20190123 3

Extracellular vesicles (EVs) are important mediators of cell-cell communication due to their cargo content of proteins, lipids, and RNAs. We previously reported that small EVs (SEVs) called exosomes promote directed and random cell motility, invasion, and serum-independent growth. In contrast, larger EVs (LEVs) were not active in those assays, but might have unique functional properties. In order to identify protein cargos that may contribute to different functions of SEVs and LEVs, we used isob ...[more]

PMID: 30608700

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