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Protein abundance changes in response to high light treatment

ABSTRACT: To characterize Arabidopsis proteomics and transcritomics changes in response to high light treatment. We quantify specific transcipts and protein abundance changes under both control and high light stress conditions. We aim to investigate the causality and interconnection of transcription and protein abundance change by an in-depth analysis of protein turnover, RNA-seq and 15N spike-in quantitative proteomics.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Shoot

SUBMITTER: Lei Li

LAB HEAD: Harvey Millar

PROVIDER: PXD010888 | Pride | 2022-07-12

REPOSITORIES: Pride

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			Action	DRS
	F1_1.dat	Other
	F1_1.raw	Raw
	F1_10.dat	Other
	F1_10.raw	Raw
	F1_11.dat	Other

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Publications

Enzymes degraded under high light maintain proteostasis by transcriptional regulation in <i>Arabidopsis</i>.

Li Lei L Duncan Owen O Ganguly Diep R DR Lee Chun Pong CP Crisp Peter A PA Wijerathna-Yapa Akila A Salih Karzan K Trösch Josua J Pogson Barry J BJ Millar A Harvey AH

Proceedings of the National Academy of Sciences of the United States of America 20220512 20

Photoinhibitory high light stress in Arabidopsis leads to increases in markers of protein degradation and transcriptional up-regulation of proteases and proteolytic machinery, but proteostasis is largely maintained. We find significant increases in the in vivo degradation rate for specific molecular chaperones, nitrate reductase, glyceraldehyde-3 phosphate dehydrogenase, and phosphoglycerate kinase and other plastid, mitochondrial, peroxisomal, and cytosolic enzymes involved in redox shuttles. C ...[more]

PMID: 35549553

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Project description:To characterize Arabidopsis proteomics and transcritomics changes in response to high light treatment. We quantify specific transcipts and protein abundance changes under both control and high light stress conditions. We aim to investigate the causality and interconnection of transcription and protein abundance change by an in-depth analysis of protein turnover, RNA-seq and 15N spike-in quantitative proteomics.

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