Dataset Information

Zebrafish 24hpf whole embryo MZezh2mutant

ABSTRACT: Polycomb group (PcG) proteins are transcriptional repressors important to maintain cell identity during embryonic development. Ezh2, the catalytic subunit of the Polycomb Repressive Complex 2, is responsible for placing the epigenetic repressive mark histone H3 lysine 27 trimethylation (H3K27me3). In contrast to results in mouse models, zebrafish embryos mutant for both maternal and zygotic ezh2 (MZezh2) can form a normal body plan at 1 day post fertilization (dpf) but die at 2 dpf, exhibiting pleiotropic phenotypes. To elucidate the specificity of PcG-mediated repression during early zebrafish development, we conducted in depth analysis of the transcriptome, epigenome, and proteome of the MZezh2 mutant embryos at 1 dpf. We found that, despite modifications in the epigenetic landscape, transcriptome and proteome analysis revealed only minor changes in gene and protein expression levels.

INSTRUMENT(S): LTQ Orbitrap, Orbitrap Fusion

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo

SUBMITTER: Leonie Kamminga

LAB HEAD: Leonie Kamminga

PROVIDER: PXD010922 | Pride | 2019-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20171003_Fusion1_SA_PJ_LK_ZF24H_EZH2mutant_MZ_Proteome_Faspsax_pH11_1.raw	Raw
	20171003_Fusion1_SA_PJ_LK_ZF24H_EZH2mutant_MZ_Proteome_Faspsax_pH11_2.raw	Raw
	20171003_Fusion1_SA_PJ_LK_ZF24H_EZH2mutant_MZ_Proteome_Faspsax_pH11_3.raw	Raw
	20171003_Fusion1_SA_PJ_LK_ZF24H_WT_Proteome_Faspsax_pH11_1.raw	Raw
	20171003_Fusion1_SA_PJ_LK_ZF24H_WT_Proteome_Faspsax_pH11_2.raw	Raw

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Publications

Maintenance of spatial gene expression by Polycomb-mediated repression after formation of a vertebrate body plan.

Rougeot Julien J Chrispijn Naomi D ND Aben Marco M Elurbe Dei M DM Andralojc Karolina M KM Murphy Patrick J PJ Jansen Pascal W T C PWTC Vermeulen Michiel M Cairns Bradley R BR Kamminga Leonie M LM

Development (Cambridge, England) 20190930 19

Polycomb group (PcG) proteins are transcriptional repressors that are important regulators of cell fate during embryonic development. Among them, Ezh2 is responsible for catalyzing the epigenetic repressive mark H3K27me3 and is essential for animal development. The ability of zebrafish embryos lacking both maternal and zygotic <i>ezh2</i> to form a normal body plan provides a unique model for comprehensively studying Ezh2 function during early development in vertebrates. By using a multi-omics a ...[more]

PMID: 31488564

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