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Duck liver TMT - Proteome and microbiota analysis reveals alterations of liver-gut axis under different stocking density of Peking ducks

ABSTRACT: The aim of this project is to investigate the effect of stocking density on duck liver proteome.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Anas Platyrhynchos

TISSUE(S): Liver

SUBMITTER: Yuqin Wu

LAB HEAD: Yuqin Wu

PROVIDER: PXD010952 | Pride | 2018-11-04

REPOSITORIES: Pride

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Publications

Proteome and microbiota analysis reveals alterations of liver-gut axis under different stocking density of Peking ducks.

Wu Yuqin Y Li Jianhui J Qin Xin X Sun Shiqiang S Xiao Zhibin Z Dong Xiaoyu X Shahid Muhammad Suhaib MS Yin Dafei D Yuan Jianmin J

PloS one 20181026 10

This study aimed to determine the impact of stocking density on the liver proteome and cecal microbiota of Peking ducks. A total of 1,200 21-day-old ducks were randomly assigned to 5 stocking density groups of 5, 6, 7, 8 and 9 ducks/m2, with 6 replicates for each group. At 40 days of age, duck serum and pectorals were collected for biochemical tests; liver and cecal contents of ducks were gathered for proteome and microbiota analysis, respectively. Serum MDA increased while pectorals T-AOC reduc ...[more]

PMID: 30365498

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Proteome and microbiota analysis reveals alterations of liver-gut axis under different stocking density of Peking ducks.

Project description:This study aimed to determine the impact of stocking density on the liver proteome and cecal microbiota of Peking ducks. A total of 1,200 21-day-old ducks were randomly assigned to 5 stocking density groups of 5, 6, 7, 8 and 9 ducks/m2, with 6 replicates for each group. At 40 days of age, duck serum and pectorals were collected for biochemical tests; liver and cecal contents of ducks were gathered for proteome and microbiota analysis, respectively. Serum MDA increased while pectorals T-AOC reduced linearly with enhancing stocking density. Duck lipid metabolism was altered under different stocking density as well. Serum LDL-C increased linearly with increasing stocking density. Proteome analysis revealed fatty acid biosynthesis proteins such as acyl-CoA synthetase family member 2 and fatty acid oxidation related proteins including acyl-CoA dehydrogenase long chain and acyl-coenzyme A oxidase were enriched in high stocking density group. Additionally, high stocking density increased oxidative response associated proteins such as DDRGK domain containing 1. Furthermore, increasing stocking density diminished proteins of anti-oxidant capacity including regucalcin and catalase. 16S rDNA analysis revealed that higher stocking density was accompanied with decreased microbial diversity, as well as depletion of anti-inflammatory bacterial taxa, including Bacteroidales, Butyricimonas and Alistipe. Besides, reduced bile acid metabolism-associated bacteria such as Ruminococcaceae, Clostridiales and Desulfovibrionaceae were found in the high-density group. Both proteome and 16S rDNA results showed inflammation and chronic liver disease trend in the high-density group, which suggests the involvement of the liver-gut axis in oxidative stress.

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