Dataset Information

A conserved atypical MFS family member orchestrates a subset of O-glycosylation to facilitate macrophage dissemination and tissue invasion

ABSTRACT: Aberrant display of the truncated core1 O-glycan T-antigen is a common feature of human cancer cells that correlates with metastasis. Here we show that T-antigen in Drosophila melanogaster macrophages is involved in their developmentally programmed tissue invasion. Higher macrophage T-antigen levels require an atypical major facilitator superfamily (MFS) member that we named Minerva which enables macrophage dissemination and invasion. We characterize for the first time the T and Tn glycoform O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva increases the presence of T-antigen on protein pathways previously linked to cancer, most strongly on the protein sulfhydryl oxidase Qsox1 which we show is required for macrophage invasion. Minerva’s vertebrate ortholog, MFSD1, rescues the minerva mutant’s migration and T-antigen glycosylation defects. We thus identify a key conserved regulator that orchestrates O-glycosylation on a protein subset to activate a program governing migration steps important for both development and cancer metastasis

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Sergey Vakhrushev

LAB HEAD: Daria E. Siekhaus

PROVIDER: PXD011045 | Pride | 2019-04-01

REPOSITORIES: Pride

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			Action	DRS
	180123_Fusion_LC2_NewObj_Daria_Drosophila_170118_2h_ETD_HCD27_90_Jacalin_SPEpH_1.raw	Raw
	180123_Fusion_LC2_NewObj_Daria_Drosophila_170118_2h_ETD_HCD27_90_Jacalin_SPEpH_2.raw	Raw
	180123_Fusion_LC2_NewObj_Daria_Drosophila_170118_2h_ETD_HCD27_90_Jacalin_SPEpH_3.raw	Raw
	180123_Fusion_LC2_NewObj_Daria_Drosophila_170118_2h_ETD_HCD27_90_Jacalin_SPEpH_4.raw	Raw
	180123_Fusion_LC2_NewObj_Daria_Drosophila_170118_2h_ETD_HCD27_90_Jacalin_SPEpH_5.raw	Raw

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Publications

A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion.

Valoskova Katarina K Biebl Julia J Roblek Marko M Emtenani Shamsi S Gyoergy Attila A Misova Michaela M Ratheesh Aparna A Reis-Rodrigues Patricia P Shkarina Kateryna K Larsen Ida Signe Bohse ISB Vakhrushev Sergey Y SY Clausen Henrik H Siekhaus Daria E DE

eLife 20190326

Aberrant display of the truncated core1 O-glycan T-antigen is a common feature of human cancer cells that correlates with metastasis. Here we show that T-antigen in <i>Drosophila melanogaster</i> macrophages is involved in their developmentally programmed tissue invasion. Higher macrophage T-antigen levels require an atypical major facilitator superfamily (MFS) member that we named Minerva which enables macrophage dissemination and invasion. We characterize for the first time the T and Tn glycof ...[more]

PMID: 30910009

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