Dataset Information

Mass spectrometric analysis of proteolytic cleavage related to ArtA and PssA from Haloferax volcanii

ABSTRACT: The S-layer glycoprotein (SLG) of Haloferax volcanii has been shown to be processed and C-terminally linked to a lipid in an archaeosortase A (ArtA)-dependent manner. A C-terminal tripartite structure including a PGF motif is required for the processing of SLG as well as other substrates (Abdul Halim et al., 2013 Mol Microbiol; Abdul Halim et al., 2016 J Bact; Abdul Halim et al., 2017 J Bact; Abdul Halim et al., 2018 Mol Microbiol). In order to gain insights into the mechanism of processing, different cellular fractions (culture supernatant, membrane) from different strains (WT, ArtA KO mutant, ArtA active site mutants, PssA KO mutant) have been analyzed. C-terminal peptides of SLG as well as peptides spanning the PGF motiv were only identified in the mutants lacking processing activity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Stefan Schulze

LAB HEAD: Mechthild Pohlschroder

PROVIDER: PXD011050 | Pride | 2020-05-11

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	Merged_results_GluC.csv	Csv
	Merged_results_GluC.csv.u.json	Csv
	Merged_results_trypsin.csv	Csv
	Merged_results_trypsin.csv.u.json	Csv
	StS_FASP-RR01_05052017.raw	Raw

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Publications

The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics.

Schulze Stefan S Adams Zachary Z Cerletti Micaela M De Castro Rosana R Ferreira-Cerca Sébastien S Fufezan Christian C Giménez María Inés MI Hippler Michael M Jevtic Zivojin Z Knüppel Robert R Legerme Georgio G Lenz Christof C Marchfelder Anita A Maupin-Furlow Julie J Paggi Roberto A RA Pfeiffer Friedhelm F Poetsch Ansgar A Urlaub Henning H Pohlschroder Mechthild M

Nature communications 20200619 1

While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comp ...[more]

PMID: 32561711

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