Proteomics

Dataset Information

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ITRAQ dataset of Pseudomonas aeruginosa PAO1 following dially disulfide treatment


ABSTRACT: To establish the effect of DADS on the P. aeruginosa proteome, 50-mL LB medium samples were inoculated at 108 CFU/mL with exponential growth phase P. aeruginosa PAO1. DADS was then added at a concentration of 0 (control) or 0.64 mg/mL, in triplicate. The six experimental groups were incubated for 5 h in a water bath shaker at 37 ºC with a shaking rate of 180 rpm. Cells from the three control groups and three DADS treatment groups were then sampled and centrifuged. The cell precipitate from each experiment group was snap-frozen at -80 ºC.

INSTRUMENT(S): TripleTOF 5600, LTQ

ORGANISM(S): Pseudomonas Aeruginosa Pao1

TISSUE(S): Whole Body

SUBMITTER: Wen-Ru Li  

LAB HEAD: Wen-Ru Li

PROVIDER: PXD011144 | Pride | 2019-01-24

REPOSITORIES: Pride

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Publications

Diallyl Disulfide From Garlic Oil Inhibits <i>Pseudomonas aeruginosa</i> Quorum Sensing Systems and Corresponding Virulence Factors.

Li Wen-Ru WR   Ma Yong-Kai YK   Xie Xiao-Bao XB   Shi Qing-Shan QS   Wen Xia X   Sun Ting-Li TL   Peng Hong H  

Frontiers in microbiology 20190107


Previously, we determined that diallyl disulfide (DADS) from garlic oil can inhibit <i>Pseudomonas aeruginosa</i> PAO1 pathogenic factors by inactivating the transcription of key genes from three quorum sensing (QS) systems (<i>las</i>, <i>rhl</i>, and <i>pqs</i>) based on the effects of DADS on growth, virulence factor production (elastase, pyocyanin, biofilm, and swarming motility), and RNA transcription (real-time q-PCR). To further investigate the mechanisms underlying the inhibition of the  ...[more]

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