Proteomics

Dataset Information

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Human FZD6 phosphorylation LC-MS/MS

ABSTRACT: Frizzleds (FZD) function as receptors for secreted lipoglycoproteins of the Wingless/Int-1 (WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are classified as G protein-coupled receptors (GPCRs), which control a wide variety of physiological functions. GPCRs are well known to be regulated by phosphorylation, which can lead to specification of downstream signaling or receptor desensitization. The role and underlying mechanisms of phosphorylation of FZDs remains largely unexplored. Here, we investigated the phosphorylation of human FZD6. In order to assess FZD6 phosphorylation experimentally, we employed mass spectrometry. HEK293 cells expressing FZD6-GFP were treated with either control, WNT-3A or WNT-5A containing conditioned medium for 30 min and processed for LC-MS/MS analysis. Six phosphorylated serine residues were detected in the C-terminus of FZD6 − S592, S620, S629, S648, S653 and S656.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Kidney Cell Line

SUBMITTER: David Potesil  

LAB HEAD: Vishnu M Dhople

PROVIDER: PXD011173 | Pride | 2018-10-18

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
131003_OV1_VMD_KS_Fzd6_3a_PO4.mgf Mgf
131003_OV1_VMD_KS_Fzd6_3a_PO4.msf Msf
131003_OV1_VMD_KS_Fzd6_3a_PO4.mzXML Mzxml
131003_OV1_VMD_KS_Fzd6_3a_PO4.pep.xml Pepxml
131003_OV1_VMD_KS_Fzd6_3a_PO4.prot.xml Xml
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Publications

Dishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.

Strakova Katerina K   Kowalski-Jahn Maria M   Gybel Tomas T   Valnohova Jana J   Dhople Vishnu M VM   Harnos Jakub J   Bernatik Ondrej O   Ganji Ranjani Sri RS   Zdrahal Zbynek Z   Mulder Jan J   Lindskog Cecilia C   Bryja Vitezslav V   Schulte Gunnar G  

The Journal of biological chemistry 20181011 48

Frizzleds (FZDs) are receptors for secreted lipoglycoproteins of the Wingless/Int-1 (WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are G protein-coupled receptors (GPCRs), which are well known to be regulated by phosphorylation, leading to specific downstream signaling or receptor desensitization. The role and underlying mechanisms of FZD phosphorylation remain largely unexplored. Here, we investigated the phosphorylation of human FZD<sub>6</su  ...[more]

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