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Intact N-glycopeptides in Serum Haptoglobin between HCC and Cirrhosis

ABSTRACT: A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jianhui Zhu

LAB HEAD: David M. Lubman

PROVIDER: PXD011239 | Pride | 2018-10-25

REPOSITORIES: Pride

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Differential Quantitative Determination of Site-Specific Intact N-Glycopeptides in Serum Haptoglobin between Hepatocellular Carcinoma and Cirrhosis Using LC-EThcD-MS/MS.

Zhu Jianhui J Chen Zhengwei Z Zhang Jie J An Mingrui M Wu Jing J Yu Qing Q Skilton St John SJ Bern Marshall M Ilker Sen K K Li Lingjun L Lubman David M DM

Journal of proteome research 20181029 1

Intact N-glycopeptide analysis remains challenging due to the complexity of glycopeptide structures, low abundance of glycopeptides in protein digests, and difficulties in data interpretation/quantitation. Herein, we developed a workflow that involved advanced methodologies, the EThcD-MS/MS fragmentation method and data interpretation software, for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma ( ...[more]

PMID: 30370771

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