Dataset Information

Alteration of Proteomes in First-generation Cultures of Bacillus pumilus Spores Exposed to Outer Space

ABSTRACT: Bacillus pumilus SAFR-032 was originally isolated from the Jet Propulsion Lab Spacecraft Assembly Facility and thoroughly characterized for its enhanced resistance to UV irradiation and oxidative stress. This unusual resistance of SAFR-032 is of particular concern in the context of planetary protection and calls for development of novel disinfection techniques to prevent extraterrestrial contamination. Previously, spores of SAFR-032 were exposed for 18 months to a variety of space conditions on board the International Space Station to investigate its resistance to Mars conditions and space travel. Here, proteomic characterization of vegetative SAFR-032 cells from space-surviving spores is presented in comparison to a ground control. Vegetative cells of the first passage were processed and subjected to quantitative proteomics using tandem mass tags. Approximately 60% of all proteins encoded by SAFR-032 were identified and 301 proteins were differentially expressed among the strains. The functional analysis of differentially expressed proteins revealed the downregulation of proteins related to carbohydrate transport/metabolism and energy production/conversion, which was validated by enzymatic assays. The same space-surviving strains showed upregulation of proteins related to competitive growth and stress response. Observed protein profiles provide insights into the possible molecular mechanisms of B. pumilus SAFR-032 to adapt and resist extreme extraterrestrial environments.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacillus Pumilus (strain Safr-032)

TISSUE(S): Vegetative Cell

SUBMITTER: Abby Chiang

LAB HEAD: Markus Kalkum

PROVIDER: PXD011292 | Pride | 2019-05-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 50

Publications

Alteration of Proteomes in First-Generation Cultures of Bacillus pumilus Spores Exposed to Outer Space.

Chiang Abby J AJ Malli Mohan Ganesh Babu GB Singh Nitin K NK Vaishampayan Parag A PA Kalkum Markus M Venkateswaran Kasthuri K

mSystems 20190611 4

<i>Bacillus pumilus</i> SAFR-032 was originally isolated from the Jet Propulsion Lab Spacecraft Assembly Facility and thoroughly characterized for its enhanced resistance to UV irradiation and oxidative stress. This unusual resistance of SAFR-032 is of particular concern in the context of planetary protection and calls for development of novel disinfection techniques to prevent extraterrestrial contamination. Previously, spores of SAFR-032 were exposed for 18 months to a variety of space conditi ...[more]

PMID: 31186338

Similar Datasets

Project description:Abstract Background The extraordinarily resistant bacterium Deinococcus radiodurans withstands harsh environmental conditions present in outer space. Deinococcus radiodurans was exposed for one year outside the International Space Station within Tanpopo orbital mission to investigate microbial survival and space travel. In addition, a ground-based simulation experiment with conditions, mirroring those from Low Earth orbit, was performed. Methods We monitored Deinococcus radiodurans cells during early stage of recovery after Low Earth orbit exposure using electron microscopy tools. Furthermore, proteomic, transcriptomic and metabolomic analyses were performed to identify molecular mechanisms responsible for the survival of Deinococcus radiodurans in Low Earth orbit. Results D. radiodurans cells exposed to low Earth orbit conditions do not exhibit any morphological damage. However, an accumulation of numerous outer-membrane associated vesicles was observed. On levels of proteins and transcripts, a multifaceted response was detected to alleviate cell stress. The UvrABC endonuclease excision repair mechanism was triggered to cope with DNA damage. Defense against reactive oxygen species is mirrored by the increased abundance of catalases and is accompanied by the increased abundance of putrescine which works as scavenging molecule. In addition, several proteins and mRNAs, responsible for regulatory and transporting functions showed increased abundances. The decrease in primary metabolites indicate alternations in the energy status, which is needed to repair damaged molecules. Conclusion Low Earth orbit induced molecular rearrangements trigger multiple components of metabolic stress response and regulatory networks in exposed microbial cells. Presented results show that the non-sporulating bacterium Deinococcus radiodurans survived long-term Low Earth orbit exposure if wavelength below 200 nm are not present, which mirrors the UV spectrum of Mars, where CO2 effectively provides a shield below 190 nm. These results should be considered in the context of planetary protection concerns and the development of new sterilization techniques for future space missions.

			Action	DRS
	BpumilusTMT1v2comb.msf	Msf
	BpumilusTMT1v2comb.pep.xml	Pepxml
	ac170303of007.raw	Raw
	ac170303of009.raw	Raw
	ac170303of011.raw	Raw