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High-density chemical cross-linking for modeling protein interactions

ABSTRACT: We combine several orthogonal cross-linking chemistries as well as improvements in search algorithms, statistical analysis and computational cost to achieve coverage of one unique cross-linked position pair for every 7 amino acids at a 1% false discovery rate. This is accomplished without any peptide-level fractionation, enrichment, or isotopic labeling. We apply our methods to model the complex between a carbonic anhydrase (CA) and its protein inhibitor, as well as the yeast proteasome

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine) Escherichia Coli Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Julian Mintseris

LAB HEAD: Steven P. Gygi

PROVIDER: PXD011296 | Pride | 2019-12-06

REPOSITORIES: Pride

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			Action	DRS
	REV9mix.fasta	Fasta
	REVPrtsmRpn11.fasta	Fasta
	REVmICA.fasta	Fasta
	t02630_150120_9mix_3hr_EDC.raw	Raw
	t02630_150120_9mix_3hr_EDC.tgz	Other

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