High-density chemical cross-linking for modeling protein interactions
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ABSTRACT: We combine several orthogonal cross-linking chemistries as well as improvements in search algorithms, statistical analysis and computational cost to achieve coverage of one unique cross-linked position pair for every 7 amino acids at a 1% false discovery rate. This is accomplished without any peptide-level fractionation, enrichment, or isotopic labeling. We apply our methods to model the complex between a carbonic anhydrase (CA) and its protein inhibitor, as well as the yeast proteasome
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine) Escherichia Coli Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: 
Julian Mintseris
LAB HEAD: Steven P. Gygi
PROVIDER: PXD011296 | Pride | 2019-12-06
REPOSITORIES: Pride
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