Proteomics

Dataset Information

0

Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions

ABSTRACT: The precise interplay between the mRNA codon and the tRNA anticodon is crucial for ensuring efficient and accurate translation by the ribosome. The insertion of RNA nucleobase derivatives in the mRNA allowed us to modulate the stability of the codon-anticodon interaction in the decoding site of bacterial and eukaryotic ribosomes, allowing an in-depth analysis of codon recognition. In addition to a quantitative analysis of the protein products that are formed in dependence of the modified codons, the interpretation of these RNA nucleobase derivatives by the ribosomal decoding site was also determined. For each modification, the translated peptides from the bacterial and eukaryotic systems were purified and analyzed by mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Thomas Hoernes  

LAB HEAD: Matthias David Erlacher

PROVIDER: PXD011311 | Pride | 2018-11-21

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
TH1-PAA.msf Msf
TH1-PAA.raw Raw
TH1-PAA.xlsx Xlsx
TH10_UZeU.msf Msf
TH10_UZeU.raw Raw
Items per page:
1 - 5 of 48
altmetric image

Publications

The precise interplay between the mRNA codon and the tRNA anticodon is crucial for ensuring efficient and accurate translation by the ribosome. The insertion of RNA nucleobase derivatives in the mRNA allowed us to modulate the stability of the codon-anticodon interaction in the decoding site of bacterial and eukaryotic ribosomes, allowing an in-depth analysis of codon recognition. We found the hydrogen bond between the N<sup>1</sup> of purines and the N<sup>3</sup> of pyrimidines to be sufficien  ...[more]

Similar Datasets

Proteomics Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions
2018-11-21 | PXD011301 | Pride
Transcriptomics Optimization of codon translation rates via tRNA modifications maintains proteome integrity
2015-06-04 | E-GEOD-67387 | biostudies-arrayexpress
Transcriptomics Optimization of codon translation rates via tRNA modifications maintains proteome integrity
2015-06-04 | GSE67387 | GEO
Transcriptomics Oligodendrocyte differentiation alters tRNA modifications and codon-dependent mRNA decay [RiboSeq]
2022-08-08 | GSE182810 | GEO
Transcriptomics Oligodendrocyte differentiation alters tRNA modifications and codon-dependent mRNA decay [DecaySeq]
2022-08-08 | GSE182809 | GEO
Genomics Oligodendrocyte differentiation alters tRNA modifications and codon-dependent mRNA decay [QuantMseq]
2022-08-08 | GSE182808 | GEO
Genomics Oligodendrocyte differentiation alters tRNA modifications and codon-dependent mRNA decay [QuantM-seq II]
2022-08-08 | GSE198271 | GEO
Other Analysing the effect of cellular energy levels on codon-specific ribosome occupancy in vivo
2024-03-08 | GSE216524 | GEO
Other The Ccr4-Not complex monitors the translating ribosome for codon optimality
2020-04-16 | GSE144250 | GEO
Other The Ccr4-Not complex monitors the translating ribosome for codon optimality
2020-04-16 | GSE131408 | GEO