Dataset Information

MCF-7 Flag-need8 IP-MS

ABSTRACT: To allow the proteomic identification of proteins modified with Nedd8, we firstly employed affinity purification and mass spectrometry to interrogate Nedd8 interactome.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: zhiqiang peng

LAB HEAD: Ping Xie

PROVIDER: PXD011368 | Pride | 2022-04-01

REPOSITORIES: Pride

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Publications

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Xie Ping P Peng Zhiqiang Z Chen Yujiao Y Li Hongchang H Du Mengge M Tan Yawen Y Zhang Xin X Lu Zhe Z Cui Chun-Ping CP Liu Cui Hua CH He Fuchu F Zhang Lingqiang L

Cell research 20201209 3

PTEN tumor suppressor opposes the PI3K/Akt signaling pathway in the cytoplasm and maintains chromosomal integrity in the nucleus. Nucleus-cytoplasm shuttling of PTEN is regulated by ubiquitylation, SUMOylation and phosphorylation, and nuclear PTEN has been proposed to exhibit tumor-suppressive functions. Here we show that PTEN is conjugated by Nedd8 under high glucose conditions, which induces PTEN nuclear import without effects on PTEN stability. PTEN neddylation is promoted by the XIAP ligase ...[more]

PMID: 33299139

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			Action	DRS
	W8007GI_11.msf	Msf
	W8007GI_11.raw	Raw
	W8007GI_21.msf	Msf
	W8007GI_21.raw	Raw