Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Epithelial Cell
SUBMITTER: Syed Mukhtar Ahmed
LAB HEAD: Ian G. Macara
PROVIDER: PXD011370 | Pride | 2018-12-05
REPOSITORIES: Pride
Items per page: 1 - 5 of 21 |
Nature communications 20181203 1
The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior to fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous exocyst subunits with sfGFP or Halo using Cas9 gene-editing, to create single and double knock-in lines of mammary epithelial cells, and interrogated exocyst dynamics by high-speed imaging and correlation spectroscopy. We discovered that mammalian exocyst is co ...[more]