Dataset Information

Isolation of spleen lymph during leukemia development

ABSTRACT: Here we asked whether infiltration of leukemic blasts initiated a response that could be detected in the interstitial fluid phase of the spleen in a rat model known to mimic human acute myeloid leukemia (AML). By cannulating efferent lymphatic vessels, we were able to monitor the response of the spleen microenvironment during leukemia development. Flow cytometric analysis of lymphocytes isolated from spleen lymph showed increased STAT3 and CREB signaling, and proteins related to these pathways were identified with a different profile in leukemic when compared with control spleen lymph. Additionally, SPARC-like 1 protein, recently identified as a promoter of AML cell growth and a biomarker of patient outcome, was locally produced in the spleen and upregulated in the leukemic setting. Thus, interstitial fluid, and its surrogate efferent lymph, can be used to provide unique information about spleen responses and substances released to the general circulation during leukemia development.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Lymph

DISEASE(S): Acute Leukemia

SUBMITTER: Kenneth Finne

LAB HEAD: Helge Wiig

PROVIDER: PXD011399 | Pride | 2023-06-23

REPOSITORIES: Pride

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			Action	DRS
	20150825_Eli_10ug_10_4ul.raw	Raw
	20150825_Eli_10ug_13_4ul.raw	Raw
	20150825_Eli_10ug_16_4ul.raw	Raw
	20150825_Eli_10ug_1_4ul.raw	Raw
	20150825_Eli_10ug_203_4ul.raw	Raw

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Isolation of lymph shows dysregulation of STAT3 and CREB pathways in the spleen and liver during leukemia development in a rat model.

Steinskog Eli Sihn Samdal ESS Finne Kenneth K Enger Marianne M Helgeland Lars L Iversen Per Ole PO McCormack Emmet E Wiig Helge H Tenstad Olav O

Microcirculation (New York, N.Y. : 1994) 20230209 2-3

<h4>Background and aims</h4>Acute myeloid leukemia (AML) is a heterogeneous malignant condition characterized by massive infiltration of poorly differentiated white blood cells in the blood stream, bone marrow, and extramedullary sites. During leukemic development, hepatosplenomegaly is expected to occur because large blood volumes are continuously filtered through these organs. We asked whether infiltration of leukemic blasts initiated a response that could be detected in the interstitial fluid ...[more]

PMID: 36702790

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