Dataset Information

Quantitative analyses reveal novel roles for N-glycosylation in a major enteric bacterial pathogen

ABSTRACT: In eukaryotes glycosylation plays a role in proteome stability, protein quality control and modulating protein function, however, similar studies in bacteria are lacking. Here, we investigate the role of general protein glycosylation systems in bacteria using the enteropathogen Campylobacter jejuni as a well-defined example. By using a quantitative proteomics strategy, we were able to monitor changes in the C. jejuni proteome when glycosylation is disrupted. We demonstrate that in C. jejuni, N-glycosylation is essential to maintain proteome stability and protein quality control. These findings guided us to investigate the role of N-glycosylation in modulating bacterial cellular activities. In glycosylation deficient C. jejuni, the multidrug efflux pump and electron transport pathways were significantly impaired. In vivo, we demonstrate that fully glycosylation deficient C. jejuni were unable to colonise its natural avian host. These results provide the first evidence of a link between proteome stability and complex functions via a bacterial general glycosylation system.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni Nctc 11168 = Atcc 700819

SUBMITTER: Sherif Abouelhadid

LAB HEAD: Sherif Abouelhadid

PROVIDER: PXD011452 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PR486_SA_TMT_20180606-1.msf	Msf
	PR486_SA_TMT_20180606.raw	Raw

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Publications

Quantitative Analyses Reveal Novel Roles for N-Glycosylation in a Major Enteric Bacterial Pathogen.

Abouelhadid Sherif S North Simon J SJ Hitchen Paul P Vohra Prerna P Chintoan-Uta Cosmin C Stevens Mark M Dell Anne A Cuccui Jon J Wren Brendan W BW

mBio 20190423 2

In eukaryotes, glycosylation plays a role in proteome stability, protein quality control, and modulating protein function; however, similar studies in bacteria are lacking. Here, we investigate the roles of general protein glycosylation systems in bacteria using the enteropathogen Campylobacter jejuni as a well-defined example. By using a quantitative proteomic strategy, we were able to monitor changes in the C. jejuni proteome when glycosylation is disrupted. We demonstrate that i ...[more]

PMID: 31015322

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Dataset Information

Quantitative analyses reveal novel roles for N-glycosylation in a major enteric bacterial pathogen

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Publications

Quantitative Analyses Reveal Novel Roles for <i>N-</i>Glycosylation in a Major Enteric Bacterial Pathogen.

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