Dataset Information

Protein turnover rate along the gastrointestinal tract is coordinated by the spatial location and the microbiota

ABSTRACT: The gastrointestinal tract is covered by a single layer of epithelial cells that, together with the mucus layers, protect the underlying tissue from bacterial invasion. The epithelium has one of the highest turnover rates in the body, renewing every 4-5 days. Using stable isotope labelling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the turnover rate of 3041 and the expression of 5012 intestinal epithelial cell proteins, analyzed under conventional and germ-free conditions across five different segments in mouse intestine. The median protein half-life was shorter in small intestine compared to colon, ranging from 3.5 to 4.2 days. Differences in protein turnover rates along the intestinal tract can be explained by distinct physiological functions and site-specific immune responses between the small and large intestine. Absence of microflora resulted in increased protein half-life by approximately one day.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Colon, Gastrointestinal Mucosa, Small Intestine

DISEASE(S): Disease Free

SUBMITTER: Liisa Arike

LAB HEAD: Liisa Arike

PROVIDER: PXD011457 | Pride | 2020-01-24

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	130420_03_QEx_LA_Fe11_pH3.raw	Raw
	130420_04_QEx_LA_Fe11_pH4.raw	Raw
	130420_05_QEx_LA_Fe11_pH5.raw	Raw
	130420_06_QEx_LA_Fe11_pH6.raw	Raw
	130420_07_QEx_LA_Fe11_pH8.raw	Raw

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Publications

Protein Turnover in Epithelial Cells and Mucus along the Gastrointestinal Tract Is Coordinated by the Spatial Location and Microbiota.

Arike Liisa L Seiman Andrus A van der Post Sjoerd S Rodriguez Piñeiro Ana M AM Ermund Anna A Schütte André A Bäckhed Fredrik F Johansson Malin E V MEV Hansson Gunnar C GC

Cell reports 20200128 4

The gastrointestinal tract is covered by a single layer of epithelial cells that, together with the mucus layers, protect the underlying tissue from microbial invasion. The epithelium has one of the highest turnover rates in the body. Using stable isotope labeling, high-resolution mass spectrometry, and computational analysis, we report a comprehensive dataset of the turnover of more than 3,000 and the expression of more than 5,000 intestinal epithelial cell proteins, analyzed under conventional ...[more]

PMID: 31995731

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