Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Zhijing Tan
LAB HEAD: David M. Lubman
PROVIDER: PXD011471 | Pride | 2020-05-26
REPOSITORIES: Pride
Action | DRS | |||
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Carrier1.dat | Other | |||
Carrier1.raw | Raw | |||
Carrier2.dat | Other | |||
Carrier2.raw | Raw | |||
Carrier3.dat | Other |
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Journal of proteome research 20181121 1
We have performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing, and a carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed phase spin columns. The fractionation in conjunction with the carrier/reference (C/R) proteome allowed us to detect 47 414 unique peptides derived from 6261 proteins, which provided a sufficient cove ...[more]