Dataset Information

Comparative analysis of CVF proteome composition during neoplastic transformation of cervix among females of reproductive age

ABSTRACT: The aim of this study is characterization of proteomic composition of the CVF for assessing the severity of HPV-associated intraepithelial neoplasias of the cervix among women of reproductive age.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervicovaginal Fluid

DISEASE(S): Cervical Cancer

SUBMITTER: Alexander Brzhozovskiy

LAB HEAD: Vladimir Frankevich

PROVIDER: PXD011472 | Pride | 2021-11-15

REPOSITORIES: Pride

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Publications

Label-free cervicovaginal fluid proteome profiling reflects the cervix neoplastic transformation.

Starodubtseva Natalia L NL Brzhozovskiy Alexander G AG Bugrova Anna E AE Kononikhin Alexey S AS Indeykina Maria I MI Gusakov Kiril I KI Chagovets Vitaliy V VV Nazarova Niso M NM Frankevich Vladimir E VE Sukhikh Gennady T GT Nikolaev Eugene N EN

Journal of mass spectrometry : JMS 20190801 8

Cervicovaginal fluid (CVF) is a valuable source of clinical information about the female reproductive tract in both nonpregnant and pregnant women. The aim of this study is to specify the CVF proteome at different stages of cervix neoplastic transformation by label-free quantitation approach based on liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The proteome composition of CVF from 40 women of reproductive age with human papillomavirus (HPV)-associated cervix neoplastic trans ...[more]

PMID: 31116903

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Project description:Background. MicroRNAs (miRNAs) are short (~22 nt) non-coding regulatory RNAs that control gene expression at the translational level. Deregulation of miRNA expression has been discovered in a wide variety of tumours and it is now clear that they contribute to cancer development and progression. This prompted the development of miRNA-chips for cancer diagnosis or prognosis, opening a new door to understand carcinogenesis. Cervical cancer is one of the most common cancers in women worldwide. Therefore, there is a strong need for a non-invasive, fast and efficient method to diagnose the disease. We investigated miRNA expression profiles in cervical cancer using a microarray platform developed in house containing probes for mature miRNAs. Results. We have evaluated miRNA expression profiles of a heterogeneous set of cervical tissues from 25 different patients. This set included 19 normal cervical tissues, 4 squamous cell carcinoma, 5 high-grade squamous intraepithelial lesion (HSIL) and 9 low-grade squamous intraepithelial lesion (LSIL) samples. We observed high variability in miRNA expression especially among normal cervical samples, which prevented us from obtaining a unique miRNA expression signature for this tumour type. However, miRNAs deregulation in malignant and pre-malignant cervical tissues was detected after tackling the high variability observed. We were also able to identify putative targets of relevant candidate miRNAs. Conclusions. Our results show that miRNA deregulation may play an important role in the malignant transformation of cervical squamous cells. In addition, deregulated miRNAs highlight new candidate targets allowing a better understanding of the molecular mechanism of this tumour type. In this study we used a common reference design experiment where the common reference used was a commercial RNA from normal cervix (Ambion) and the test samples were 4 pre-treatment squamous cell cervical carcinoma, 7 high-grade Squamous Intraepithelial Lesion (CINII, n=2 and CIN III, n=5) sample, 9 low-grade Squamous Intraepithelial Lesion (CIN I) samples, 19 normal cervix samples and 4 pools of normal cervix samples.

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