Proteomics

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Bacteriocin LC-MSMS

ABSTRACT: This is the first report of a bacteriocin being produced by lactobacillus acidophilus ATCC 4356. For protein identification, the ~8-kDa peptide band was removed from the acrylamide gel and digested in-gel by trypsin, and the resulting peptide fragments were extracted and analyzed by LC-MS/MS analysis. The MS data were processed using Thermo Proteome Discoverer software (v2.2) with the SEQUEST search engine. Three peptides, VAHCASQIGR (amino acids 23-32), GSAACVSYLTR (amino acids 69-79), GSAACVSYLTRHRHH (amino acids 69-83) were identified as tryptic fragments on the basis of LC-MS/MS.This is the first report of a bacteriocin being produced by lactobacillus acidophilus ATCC 4356. For protein identification, the ~8-kDa peptide band was removed from the acrylamide gel and digested in-gel by trypsin, and the resulting peptide fragments were extracted and analyzed by LC-MS/MS analysis. The MS data were processed using Thermo Proteome Discoverer software (v2.2) with the SEQUEST search engine. Three peptides, VAHCASQIGR (amino acids 23-32), GSAACVSYLTR (amino acids 69-79), GSAACVSYLTRHRHH (amino acids 69-83) were identified as tryptic fragments on the basis of LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Lactobacillus Acidophilus

SUBMITTER: Sima Modiri  

LAB HEAD: Kambiz Akbari Noghabi

PROVIDER: PXD011556 | Pride | 2020-03-24

REPOSITORIES: pride

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Publications

Multifunctional Acidocin 4356 Combats <i>Pseudomonas aeruginosa</i> through Membrane Perturbation and Virulence Attenuation: Experimental Results Confirm Molecular Dynamics Simulation.

Modiri Sima S   Kasra Kermanshahi Rouha R   Soudi Mohammad Reza MR   Arab Seyed Shahriar SS   Khammari Anahita A   Cousineau Benoit B   Vali Hojatollah H   Zahiri Hossein Shahbani HS   Noghabi Kambiz Akbari KA  

Applied and environmental microbiology 20200505 10

A longstanding awareness in generating resistance to common antimicrobial therapies by Gram-negative bacteria has made them a major threat to global health. The application of antimicrobial peptides as a therapeutic agent would be a great opportunity to combat bacterial diseases. Here, we introduce a new antimicrobial peptide (∼8.3 kDa) from probiotic strain <i>Lactobacillus acidophilus</i> ATCC 4356, designated acidocin 4356 (ACD). This multifunctional peptide exerts its anti-infective ability  ...[more]

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